INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS.

Bernd Steinbach, Eugene A Sprague, Colin Schwartz, Robert Nerem

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Low density lipoprotein (LDL) transport and receptor-mediated metabolism are believed to be key factors in atherogenesis. The purpose of this study was to establish a link between LDL-internalization and steady, laminar hemodynamic shear stress by subjecting cultured bovine aortic endothelial cells (BAEC) to a known shear stress in a parallel-plate, channel flow device. The present studies establish that the application of a laminar, steady wall shear stress to confluent BAEC cultures enhances the internalization of **1**2**5I-LDL. This effect appears to not be dependent upon prior shear-induced changes in cell shape and orientation, nor is it limited to the time period during which cell geometry is changing.

Original languageEnglish (US)
Title of host publicationAmerican Society of Mechanical Engineers, Applied Mechanics Division, AMD
PublisherASME
Pages191-194
Number of pages4
Volume84
StatePublished - 1987

Fingerprint

Lipoproteins
Endothelial cells
Shear stress
Hemodynamics
Channel flow
Cell culture
Metabolism
Geometry

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Steinbach, B., Sprague, E. A., Schwartz, C., & Nerem, R. (1987). INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS. In American Society of Mechanical Engineers, Applied Mechanics Division, AMD (Vol. 84, pp. 191-194). ASME.

INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS. / Steinbach, Bernd; Sprague, Eugene A; Schwartz, Colin; Nerem, Robert.

American Society of Mechanical Engineers, Applied Mechanics Division, AMD. Vol. 84 ASME, 1987. p. 191-194.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Steinbach, B, Sprague, EA, Schwartz, C & Nerem, R 1987, INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS. in American Society of Mechanical Engineers, Applied Mechanics Division, AMD. vol. 84, ASME, pp. 191-194.
Steinbach B, Sprague EA, Schwartz C, Nerem R. INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS. In American Society of Mechanical Engineers, Applied Mechanics Division, AMD. Vol. 84. ASME. 1987. p. 191-194
Steinbach, Bernd ; Sprague, Eugene A ; Schwartz, Colin ; Nerem, Robert. / INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS. American Society of Mechanical Engineers, Applied Mechanics Division, AMD. Vol. 84 ASME, 1987. pp. 191-194
@inproceedings{cb4e41bfa21547b2b6b6ef49742b1825,
title = "INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS.",
abstract = "Low density lipoprotein (LDL) transport and receptor-mediated metabolism are believed to be key factors in atherogenesis. The purpose of this study was to establish a link between LDL-internalization and steady, laminar hemodynamic shear stress by subjecting cultured bovine aortic endothelial cells (BAEC) to a known shear stress in a parallel-plate, channel flow device. The present studies establish that the application of a laminar, steady wall shear stress to confluent BAEC cultures enhances the internalization of **1**2**5I-LDL. This effect appears to not be dependent upon prior shear-induced changes in cell shape and orientation, nor is it limited to the time period during which cell geometry is changing.",
author = "Bernd Steinbach and Sprague, {Eugene A} and Colin Schwartz and Robert Nerem",
year = "1987",
language = "English (US)",
volume = "84",
pages = "191--194",
booktitle = "American Society of Mechanical Engineers, Applied Mechanics Division, AMD",
publisher = "ASME",

}

TY - GEN

T1 - INFLUENCE OF A STEADY LAMINAR SHEAR STRESS ON THE INTERNALIZATION OF LOW DENSITY LIPOPROTEINS BY CULTURED BOVINE AORTIC ENDOTHELIAL CELLS.

AU - Steinbach, Bernd

AU - Sprague, Eugene A

AU - Schwartz, Colin

AU - Nerem, Robert

PY - 1987

Y1 - 1987

N2 - Low density lipoprotein (LDL) transport and receptor-mediated metabolism are believed to be key factors in atherogenesis. The purpose of this study was to establish a link between LDL-internalization and steady, laminar hemodynamic shear stress by subjecting cultured bovine aortic endothelial cells (BAEC) to a known shear stress in a parallel-plate, channel flow device. The present studies establish that the application of a laminar, steady wall shear stress to confluent BAEC cultures enhances the internalization of **1**2**5I-LDL. This effect appears to not be dependent upon prior shear-induced changes in cell shape and orientation, nor is it limited to the time period during which cell geometry is changing.

AB - Low density lipoprotein (LDL) transport and receptor-mediated metabolism are believed to be key factors in atherogenesis. The purpose of this study was to establish a link between LDL-internalization and steady, laminar hemodynamic shear stress by subjecting cultured bovine aortic endothelial cells (BAEC) to a known shear stress in a parallel-plate, channel flow device. The present studies establish that the application of a laminar, steady wall shear stress to confluent BAEC cultures enhances the internalization of **1**2**5I-LDL. This effect appears to not be dependent upon prior shear-induced changes in cell shape and orientation, nor is it limited to the time period during which cell geometry is changing.

UR - http://www.scopus.com/inward/record.url?scp=0023168792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023168792&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0023168792

VL - 84

SP - 191

EP - 194

BT - American Society of Mechanical Engineers, Applied Mechanics Division, AMD

PB - ASME

ER -