Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates

Janet M. Harouse, Francisco González-Scarano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Most studies looking at CD4-independent infection have used laboratory strains or their respective molecular clones. To determine whether primary human immunodeficiency virus type 1 isolates could infect CD4-negative cells, we obtained a panel of 23 clinical isolates and characterized the early steps of the viral life cycle in SK-N-MC cells, a CD4-negative, galactosylceramide- positive neuroblastoma cell line. Eight of 23 isolates established a nonproductive infection; entry and postentry restrictions were noted in the others. We were unable to correlate the infectivity for SK-N-MC cells with established biological phenotypes, such as syncytium induction, or with genetic classifications, suggesting that pantropism is an independent biological variable.

Original languageEnglish (US)
Pages (from-to)7290-7294
Number of pages5
JournalJournal of Virology
Volume70
Issue number10
StatePublished - Oct 1996
Externally publishedYes

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Human immunodeficiency virus 1
Neuroblastoma
HIV-1
cell lines
Cell Line
Infection
infection
Galactosylceramides
giant cells
cells
Giant Cells
Life Cycle Stages
life cycle (organisms)
pathogenicity
Clone Cells
clones
taxonomy
Phenotype
phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates. / Harouse, Janet M.; González-Scarano, Francisco.

In: Journal of Virology, Vol. 70, No. 10, 10.1996, p. 7290-7294.

Research output: Contribution to journalArticle

Harouse, Janet M. ; González-Scarano, Francisco. / Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates. In: Journal of Virology. 1996 ; Vol. 70, No. 10. pp. 7290-7294.
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