TY - JOUR
T1 - Induction of short-term markers of tumor promotion by organic peroxides
AU - Gimenez-conti, Irma
AU - Viaje, Aurora
AU - Chesner, Judith
AU - Conti, Claudio
AU - Slaga, Thomas J.
N1 - Funding Information:
The authors would like to thank J E.Thompson and H.L. Vaughan for the synthesis of di-m-chlorobenzoyl peroxide, Mary Lou Fendley and Christie Hoy for typing the manuscript and secretarial assistance and John Riley for table preparation. The research presented was supported by The Procter & Gamble Company, Cincinnati, OH, USA
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1991/4
Y1 - 1991/4
N2 - Experiments from different laboratories have shown that benzoyl peroxide (BzPo) and other organic peroxides are effective tumor promoters in the mouse skin two-stage carcinogenesis system. In the present paper we have studied the short-term effect of six other organic peroxides, which have not been previously assayed as skin tumor promoters. These compounds were chosen for their molecular diversity, the type of radical predicted to be formed, solubility and availability. The parameters evaluated in this study include a series of short-term markers of tumor promotion, hyperplasia, induction of dark basal keratinocytes and induction of ornithine decarboxylase activity. After single applications the biological activity of the compounds was: m-chloroperoxybenzoic acid > dl-m-methylbenzoyl peroxide > dlcumyl peroxide > O,O-t-butyl-O-(2-ethylhexyl)monoperoxycarbonate > benzoyl peroxide > di-m-chlorobenzoyl peroxide > di-t-butyl peroxide > t-butyl hydroperoxide. After multiple applications, the order of activity of the compounds was: dicumyl peroxide > di-m-methyl benzoyl peroxide > O,O-t-butyl-O-(2-ethylhexyl)monoperoxy carbonate > di-m-chloroperoxybenzoic acid > dl-m-chloro benzoyl peroxide > t-butyl hydroperoxide > benzoyl peroxide > di-t-butyl peroxide. The difference of activity among the different compounds did not seem to correlate directly with the chemical stability of the compound; it is more likely that the activity depends on different factors such as percutaneous absorption, metabolism, and the rate of free radical formation in vivo. The data presented here further support the association between free radicals and tumor promotion since all of the compounds, with the exception of one, were active in inducing the short-term markers of tumor promotion. It will also establish conditions for future tumor experiments.
AB - Experiments from different laboratories have shown that benzoyl peroxide (BzPo) and other organic peroxides are effective tumor promoters in the mouse skin two-stage carcinogenesis system. In the present paper we have studied the short-term effect of six other organic peroxides, which have not been previously assayed as skin tumor promoters. These compounds were chosen for their molecular diversity, the type of radical predicted to be formed, solubility and availability. The parameters evaluated in this study include a series of short-term markers of tumor promotion, hyperplasia, induction of dark basal keratinocytes and induction of ornithine decarboxylase activity. After single applications the biological activity of the compounds was: m-chloroperoxybenzoic acid > dl-m-methylbenzoyl peroxide > dlcumyl peroxide > O,O-t-butyl-O-(2-ethylhexyl)monoperoxycarbonate > benzoyl peroxide > di-m-chlorobenzoyl peroxide > di-t-butyl peroxide > t-butyl hydroperoxide. After multiple applications, the order of activity of the compounds was: dicumyl peroxide > di-m-methyl benzoyl peroxide > O,O-t-butyl-O-(2-ethylhexyl)monoperoxy carbonate > di-m-chloroperoxybenzoic acid > dl-m-chloro benzoyl peroxide > t-butyl hydroperoxide > benzoyl peroxide > di-t-butyl peroxide. The difference of activity among the different compounds did not seem to correlate directly with the chemical stability of the compound; it is more likely that the activity depends on different factors such as percutaneous absorption, metabolism, and the rate of free radical formation in vivo. The data presented here further support the association between free radicals and tumor promotion since all of the compounds, with the exception of one, were active in inducing the short-term markers of tumor promotion. It will also establish conditions for future tumor experiments.
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U2 - 10.1093/carcin/12.4.563
DO - 10.1093/carcin/12.4.563
M3 - Article
C2 - 2013121
AN - SCOPUS:0025803709
VL - 12
SP - 563
EP - 569
JO - Carcinogenesis
JF - Carcinogenesis
SN - 0143-3334
IS - 4
ER -