Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal

Robert E. Lanford, Patrick Kanda, Ronald C. Kennedy

    Research output: Contribution to journalArticle

    246 Scopus citations

    Abstract

    A system was developed for the analysis of protein transport to the nucleus. Carrier proteins cross-linked to synthetic peptides were microinjected into the cytoplasm of mammalian cells, and protein transport was evaluated by immunofluorescence staining of fixed cells. A 13-mer synthetic peptide containing seven amino acids homologous to SV40 T antigen was capable of inducing nuclear transport, but no transport was observed when proteins were coupled with a synthetic peptide homologous to a nuclear-transport-defective T antigen. The largest protein-peptide conjugate efficiently transported was ferritin (Mr 465,000). The rate of transport was influenced by the number of peptides per molecule of carrier protein and, to some degree, by the size of the carrier protein. Transport of some conjugates was almost complete in 15 min at room temperature.

    Original languageEnglish (US)
    Pages (from-to)575-582
    Number of pages8
    JournalCell
    Volume46
    Issue number4
    DOIs
    StatePublished - Aug 15 1986

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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