Induction of nuclear factor κB but not κB-responsive cytokine expression during myocardial reperfusion injury after neutropenia

Bysani Chandrasekar, James T. Colston, Jone Geimer, Maria D Cortez, Gregory L. Freeman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Neutrophils may contribute to myocardial ischemia/reperfusion (I/R) injury by generating reactive oxygen intermediates (ROIs). ROIs activate nuclear factor (NF)-κB, which regulates genes for cytokines with negative inotropic effects (interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF]-α). We investigated the impact of neutrophil depletion on NF-κB DNA binding activity, and expression of these cytokines during myocardial I/R injury. Male WKY rats (n = 28) received a single dose of antineutrophil antiserum (i/v). Twenty two hours later, when the peripheral blood neutrophil counts were profoundly decreased (94% reduction), the animals underwent 15 min of left anterior descending coronary artery ligation followed by reperfusion for 0.25, 0.5, 1, 2, 3, and 6 h (n = 4/group). Saline-treated animals underwent a similar protocol, and served as controls (n = 28, 4/group). Neutrophil accumulation, defined by myeloperoxidase activity, was present in controls, but not in anti-PMN antisera-treated animals (at least p < 0.05 at 1, 2, 3, and 6 h R). Despite this difference, in both saline- and antiserum-treated animals, the GSH levels were very similar and fell significantly (p < 0.0001) at 15 min R; the levels increased gradually over time. In contrast, GSSG levels rose at 15 and 30 min R (p < 0.05), and declined thereafter. NF-κB DNA binding activity increased in both groups at 15 min and again at 3 h of R. Both NF-κBp50 and p65 subunits were detected by supershift assay. In saline-injected controls both mRNA and protein for IL-1β, IL-6, and TNF-α were detected at 1 h R; levels remained high until 3 h, then fell (except IL-6, which was elevated at 6 h). In neutropenic animals, however, a significant decrease in mRNA (at least 1.7-fold, p < 0.05) as well as protein levels (at least 2.3-fold, p < 0.01) for all three cytokines was observed. Thus, while neutrophils had minimal effects on oxidative stress (GSH/GSSG) and oxidative stress-responsive NF-κB activity, they contributed significantly to myocardial cytokine expression. (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)1579-1588
Number of pages10
JournalFree Radical Biology and Medicine
Volume28
Issue number11
DOIs
StatePublished - Jun 1 2000

Fingerprint

Myocardial Reperfusion Injury
Neutropenia
Animals
Neutrophils
Cytokines
Immune Sera
Interleukin-6
Oxidative stress
Glutathione Disulfide
Reperfusion Injury
Interleukin-1
Myocardial Ischemia
Oxidative Stress
Tumor Necrosis Factor-alpha
Oxygen
Messenger RNA
Inbred WKY Rats
DNA
Peroxidase
Reperfusion

Keywords

  • Anti-PMN antiserum
  • Free radicals
  • Ischemia
  • Neutrophils
  • NF-κB
  • Proinflammatory cytokines
  • Reperfusion injury

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Induction of nuclear factor κB but not κB-responsive cytokine expression during myocardial reperfusion injury after neutropenia. / Chandrasekar, Bysani; Colston, James T.; Geimer, Jone; Cortez, Maria D; Freeman, Gregory L.

In: Free Radical Biology and Medicine, Vol. 28, No. 11, 01.06.2000, p. 1579-1588.

Research output: Contribution to journalArticle

Chandrasekar, Bysani ; Colston, James T. ; Geimer, Jone ; Cortez, Maria D ; Freeman, Gregory L. / Induction of nuclear factor κB but not κB-responsive cytokine expression during myocardial reperfusion injury after neutropenia. In: Free Radical Biology and Medicine. 2000 ; Vol. 28, No. 11. pp. 1579-1588.
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abstract = "Neutrophils may contribute to myocardial ischemia/reperfusion (I/R) injury by generating reactive oxygen intermediates (ROIs). ROIs activate nuclear factor (NF)-κB, which regulates genes for cytokines with negative inotropic effects (interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF]-α). We investigated the impact of neutrophil depletion on NF-κB DNA binding activity, and expression of these cytokines during myocardial I/R injury. Male WKY rats (n = 28) received a single dose of antineutrophil antiserum (i/v). Twenty two hours later, when the peripheral blood neutrophil counts were profoundly decreased (94{\%} reduction), the animals underwent 15 min of left anterior descending coronary artery ligation followed by reperfusion for 0.25, 0.5, 1, 2, 3, and 6 h (n = 4/group). Saline-treated animals underwent a similar protocol, and served as controls (n = 28, 4/group). Neutrophil accumulation, defined by myeloperoxidase activity, was present in controls, but not in anti-PMN antisera-treated animals (at least p < 0.05 at 1, 2, 3, and 6 h R). Despite this difference, in both saline- and antiserum-treated animals, the GSH levels were very similar and fell significantly (p < 0.0001) at 15 min R; the levels increased gradually over time. In contrast, GSSG levels rose at 15 and 30 min R (p < 0.05), and declined thereafter. NF-κB DNA binding activity increased in both groups at 15 min and again at 3 h of R. Both NF-κBp50 and p65 subunits were detected by supershift assay. In saline-injected controls both mRNA and protein for IL-1β, IL-6, and TNF-α were detected at 1 h R; levels remained high until 3 h, then fell (except IL-6, which was elevated at 6 h). In neutropenic animals, however, a significant decrease in mRNA (at least 1.7-fold, p < 0.05) as well as protein levels (at least 2.3-fold, p < 0.01) for all three cytokines was observed. Thus, while neutrophils had minimal effects on oxidative stress (GSH/GSSG) and oxidative stress-responsive NF-κB activity, they contributed significantly to myocardial cytokine expression. (C) 2000 Elsevier Science Inc.",
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AU - Freeman, Gregory L.

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N2 - Neutrophils may contribute to myocardial ischemia/reperfusion (I/R) injury by generating reactive oxygen intermediates (ROIs). ROIs activate nuclear factor (NF)-κB, which regulates genes for cytokines with negative inotropic effects (interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF]-α). We investigated the impact of neutrophil depletion on NF-κB DNA binding activity, and expression of these cytokines during myocardial I/R injury. Male WKY rats (n = 28) received a single dose of antineutrophil antiserum (i/v). Twenty two hours later, when the peripheral blood neutrophil counts were profoundly decreased (94% reduction), the animals underwent 15 min of left anterior descending coronary artery ligation followed by reperfusion for 0.25, 0.5, 1, 2, 3, and 6 h (n = 4/group). Saline-treated animals underwent a similar protocol, and served as controls (n = 28, 4/group). Neutrophil accumulation, defined by myeloperoxidase activity, was present in controls, but not in anti-PMN antisera-treated animals (at least p < 0.05 at 1, 2, 3, and 6 h R). Despite this difference, in both saline- and antiserum-treated animals, the GSH levels were very similar and fell significantly (p < 0.0001) at 15 min R; the levels increased gradually over time. In contrast, GSSG levels rose at 15 and 30 min R (p < 0.05), and declined thereafter. NF-κB DNA binding activity increased in both groups at 15 min and again at 3 h of R. Both NF-κBp50 and p65 subunits were detected by supershift assay. In saline-injected controls both mRNA and protein for IL-1β, IL-6, and TNF-α were detected at 1 h R; levels remained high until 3 h, then fell (except IL-6, which was elevated at 6 h). In neutropenic animals, however, a significant decrease in mRNA (at least 1.7-fold, p < 0.05) as well as protein levels (at least 2.3-fold, p < 0.01) for all three cytokines was observed. Thus, while neutrophils had minimal effects on oxidative stress (GSH/GSSG) and oxidative stress-responsive NF-κB activity, they contributed significantly to myocardial cytokine expression. (C) 2000 Elsevier Science Inc.

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