Induction of long-term protective effects against heterologous challenge in SIVhu-infected macaques

François Villinger, William M. Switzer, Bharat S. Parekh, Ronald A. Otten, Debra Adams, Vedapuri Shanmugam, Pavel Bostik, Ann E. Mayne, Nathaniel F. Chikkala, Harold M. McClure, Francis Novembre, Qizhi Yao, Walid Heneine, Thomas M. Folks, Aftab A. Ansari

    Research output: Contribution to journalArticlepeer-review

    14 Scopus citations

    Abstract

    A group of three rhesus macaques were inoculated with SIV isolated from a human (SIVhu) accidentally exposed and infected with SIVsm. Extensive sequence analyses of SIVhu obtained from the human and macaques following infection indicated the presence of truncated nef. Not only did nef fail to repair itself in vivo postinfection (p.i.), but instead, further mutations added additional stop codons with increasing time p.i. Infection of these animals was associated with minimal acute viral replication, followed by undetectable plasma viral loads and only intermittent PCR detection up to 5 years p.i. The three SIVhu infected and three control monkeys were then challenged with the heterologous highly pathogenic SHIV89.6p. All three controls became infected and showed rapid declines in peripheral CD4+ lymphocytes, disease, and death at 10 and 32 weeks p.i., respectively. In contrast, all three animals previously infected with SIVhu are healthy and exhibit stable CD4+ lymphocyte levels and undetectable plasma viral loads at >20 months post-SHIV89.6p challenge. Only transient, low levels of SHIV replication were noted in these animals. Whereas responses to SIVgag/pol were noted, no evidence for SIV/SHIV envelope cross-reactivity was detected by antibody or CTL analyses, suggesting that the protective immune mechanisms to the heterologous challenge isolate were most likely not directed to envelope but rather to other viral determinants. (C) 2000 Academic Press.

    Original languageEnglish (US)
    Pages (from-to)194-206
    Number of pages13
    JournalVirology
    Volume278
    Issue number1
    DOIs
    StatePublished - Dec 5 2000

    ASJC Scopus subject areas

    • Virology

    Fingerprint Dive into the research topics of 'Induction of long-term protective effects against heterologous challenge in SIVhu-infected macaques'. Together they form a unique fingerprint.

    Cite this