Induction of IL-8 by Chlamydia trachomatis through MAPK pathway rather than NF-κB pathway

Fan Chen, Wen Cheng, Saidan Zhang, Guangming Zhong, Ping Yu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Objective: To determine the signaling pathway required for Chlamydial induction of IL-8 expression in epithelial cells. Methods: The production and localization of IL-8 in Chlamydiainfected Hela 229 cells were monitored using Western blot, immunoflourescence, and ELISA. Activation of MAPK and NF- κB signaling pathways were detected by Western blot and immunoflourescence. The effect of different signaling pathways on Chlamydia-induced Il-8 was measured by experiments of chemical inhibitors. Results: IL-8 was induced by Chlamydia and was time-dependant. Chlamydial infection activated MAPK/ERK and MAPK/p38 pathways but not NF- κkB pathway. Chlamydial induction of IL-8 was blocked by small molecule inhibitors targeting the ERK and p38 pathways. Conclusion: Chlamydia-induced IL-8 in cervical epithelial cells, the natural target cell type of Chlamydia trachomatis infection, is dependent on MAPK pathway but not NF-κB pathway, which provides important information for further understanding the molecular mechanism of Chlamydia-induced inflammatory pathologies.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalJournal of Central South University (Medical Sciences)
Volume35
Issue number4
DOIs
StatePublished - Apr 1 2010

Keywords

  • Chlamydia trachomatis
  • IL-8
  • Map kinase pathway
  • NF-B pathway

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint

Dive into the research topics of 'Induction of IL-8 by Chlamydia trachomatis through MAPK pathway rather than NF-<sub>κ</sub>B pathway'. Together they form a unique fingerprint.

Cite this