Abstract
Objective: To determine the signaling pathway required for Chlamydial induction of IL-8 expression in epithelial cells. Methods: The production and localization of IL-8 in Chlamydiainfected Hela 229 cells were monitored using Western blot, immunoflourescence, and ELISA. Activation of MAPK and NF- κB signaling pathways were detected by Western blot and immunoflourescence. The effect of different signaling pathways on Chlamydia-induced Il-8 was measured by experiments of chemical inhibitors. Results: IL-8 was induced by Chlamydia and was time-dependant. Chlamydial infection activated MAPK/ERK and MAPK/p38 pathways but not NF- κkB pathway. Chlamydial induction of IL-8 was blocked by small molecule inhibitors targeting the ERK and p38 pathways. Conclusion: Chlamydia-induced IL-8 in cervical epithelial cells, the natural target cell type of Chlamydia trachomatis infection, is dependent on MAPK pathway but not NF-κB pathway, which provides important information for further understanding the molecular mechanism of Chlamydia-induced inflammatory pathologies.
Original language | English (US) |
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Pages (from-to) | 307-313 |
Number of pages | 7 |
Journal | Journal of Central South University (Medical Sciences) |
Volume | 35 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2010 |
Keywords
- Chlamydia trachomatis
- IL-8
- Map kinase pathway
- NF-B pathway
ASJC Scopus subject areas
- General Medicine