TY - JOUR
T1 - Induction of an adaptive response in human blood lymphocytes exposed to radiofrequency fields
T2 - Influence of the universal mobile telecommunication system (UMTS) signal and the specific absorption rate
AU - Zeni, Olga
AU - Sannino, Anna
AU - Romeo, Stefania
AU - Massa, Rita
AU - Sarti, Maurizio
AU - Reddy, Abishek B.
AU - Prihoda, Thomas J.
AU - Vijayalaxmi,
AU - Scarfì, Maria Rosaria
PY - 2012/8/30
Y1 - 2012/8/30
N2 - The induction of an adaptive response (AR) was examined in human peripheral blood lymphocytes exposed to non-ionizing radiofrequency fields (RF). Cells from nine healthy human volunteers were stimulated for 24. h with phytohaemagglutinin and then exposed for 20. h to an adaptive dose (AD) of a 1950. MHz RF UMTS (universal mobile telecommunication system) signal used for mobile communications, at different specific absorption rates (SAR) of 1.25, 0.6, 0.3, and 0.15. W/kg. This was followed by treatment of the cells at 48. h with a challenge dose (CD) of 100. ng/ml mitomycin C (MMC). Lymphocytes were collected at the end of the 72. h total culture period. The cytokinesis-block method was used to record the frequency of micronuclei (MN) as genotoxicity end-point. When lymphocytes from six donors were pre-exposed to RF at 0.3. W/kg SAR and then treated with MMC, these cells showed a significant reduction in the frequency of MN, compared with the cells treated with MMC alone; this result is indicative of induction of AR. The results from our earlier study indicated that lymphocytes that were stimulated for 24. h, exposed for 20. h to a 900. MHz RF GSM (global system for mobile communication) signal at 1.25. W/kg SAR and then treated with 100. ng/ml MMC, also exhibited AR. These overall data suggest that the induction of AR depends on RF frequency, type of the signal and SAR. Further characterization of RF-induced AR is in progress.
AB - The induction of an adaptive response (AR) was examined in human peripheral blood lymphocytes exposed to non-ionizing radiofrequency fields (RF). Cells from nine healthy human volunteers were stimulated for 24. h with phytohaemagglutinin and then exposed for 20. h to an adaptive dose (AD) of a 1950. MHz RF UMTS (universal mobile telecommunication system) signal used for mobile communications, at different specific absorption rates (SAR) of 1.25, 0.6, 0.3, and 0.15. W/kg. This was followed by treatment of the cells at 48. h with a challenge dose (CD) of 100. ng/ml mitomycin C (MMC). Lymphocytes were collected at the end of the 72. h total culture period. The cytokinesis-block method was used to record the frequency of micronuclei (MN) as genotoxicity end-point. When lymphocytes from six donors were pre-exposed to RF at 0.3. W/kg SAR and then treated with MMC, these cells showed a significant reduction in the frequency of MN, compared with the cells treated with MMC alone; this result is indicative of induction of AR. The results from our earlier study indicated that lymphocytes that were stimulated for 24. h, exposed for 20. h to a 900. MHz RF GSM (global system for mobile communication) signal at 1.25. W/kg SAR and then treated with 100. ng/ml MMC, also exhibited AR. These overall data suggest that the induction of AR depends on RF frequency, type of the signal and SAR. Further characterization of RF-induced AR is in progress.
KW - Adaptive response
KW - Human blood lymphocytes
KW - Micronuclei
KW - Radiofrequency fields
KW - Specific absorption rate
KW - UMTS signal
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U2 - 10.1016/j.mrgentox.2012.03.013
DO - 10.1016/j.mrgentox.2012.03.013
M3 - Article
C2 - 22525361
AN - SCOPUS:84862306527
VL - 747
SP - 29
EP - 35
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
SN - 1383-5718
IS - 1
ER -