Abstract
Purpose: To investigate the influence of cell cycle on the adaptive response (AR) induced by the exposure of human blood lymphocytes to radiofrequency fields (RF). Materials and methods: Human peripheral blood lymphocytes in G0-, G1- or S-phase of the cell cycle were exposed for 20 hours to an adaptive dose (AD) of 900 MHz RF at an average specific absorption rate of 1.25 W/kg and then treated with a challenge dose (CD) of 100 ng/ml mitomycin C (MMC). Un-exposed and sham-exposed controls as well as cells treated with MMC alone were included in the study. The incidence of micronuclei (MN) was evaluated to determine the induction of AR. Results: The results indicated that the cells which were exposed to AD of RF in G 0- and G1-phase of the cell cycle did not exhibit AR while such a response was observed when the cells were exposed to AD of RF in S-phase of the cell cycle. Conclusions: These results confirmed the observations reported in our previous investigation where AR was observed in human blood lymphocytes exposed to AD of RF in S-phase of the cell cycle and further suggested that the timing of AD exposure of RF is important to elicit AR.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 993-999 |
| Number of pages | 7 |
| Journal | International Journal of Radiation Biology |
| Volume | 87 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2011 |
| Externally published | Yes |
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Keywords
- Adaptive response
- Cell cycle
- Human blood lymphocytes
- Micronuclei
- Radiofrequency fields
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
Cite this
Induction of adaptive response in human blood lymphocytes exposed to 900 MHz radiofrequency fields : Influence of cell cycle. / Sannino, Anna; Zeni, Olga; Sarti, Maurizio; Romeo, Stefania; Reddy, Siddharth B.; Belisario, Maria Antonietta; Prihoda, Thomas J.; Vijayalaxmi; Scarfi, Maria Rosaria.
In: International Journal of Radiation Biology, Vol. 87, No. 9, 09.2011, p. 993-999.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of adaptive response in human blood lymphocytes exposed to 900 MHz radiofrequency fields
T2 - Influence of cell cycle
AU - Sannino, Anna
AU - Zeni, Olga
AU - Sarti, Maurizio
AU - Romeo, Stefania
AU - Reddy, Siddharth B.
AU - Belisario, Maria Antonietta
AU - Prihoda, Thomas J.
AU - Vijayalaxmi,
AU - Scarfi, Maria Rosaria
PY - 2011/9
Y1 - 2011/9
N2 - Purpose: To investigate the influence of cell cycle on the adaptive response (AR) induced by the exposure of human blood lymphocytes to radiofrequency fields (RF). Materials and methods: Human peripheral blood lymphocytes in G0-, G1- or S-phase of the cell cycle were exposed for 20 hours to an adaptive dose (AD) of 900 MHz RF at an average specific absorption rate of 1.25 W/kg and then treated with a challenge dose (CD) of 100 ng/ml mitomycin C (MMC). Un-exposed and sham-exposed controls as well as cells treated with MMC alone were included in the study. The incidence of micronuclei (MN) was evaluated to determine the induction of AR. Results: The results indicated that the cells which were exposed to AD of RF in G 0- and G1-phase of the cell cycle did not exhibit AR while such a response was observed when the cells were exposed to AD of RF in S-phase of the cell cycle. Conclusions: These results confirmed the observations reported in our previous investigation where AR was observed in human blood lymphocytes exposed to AD of RF in S-phase of the cell cycle and further suggested that the timing of AD exposure of RF is important to elicit AR.
AB - Purpose: To investigate the influence of cell cycle on the adaptive response (AR) induced by the exposure of human blood lymphocytes to radiofrequency fields (RF). Materials and methods: Human peripheral blood lymphocytes in G0-, G1- or S-phase of the cell cycle were exposed for 20 hours to an adaptive dose (AD) of 900 MHz RF at an average specific absorption rate of 1.25 W/kg and then treated with a challenge dose (CD) of 100 ng/ml mitomycin C (MMC). Un-exposed and sham-exposed controls as well as cells treated with MMC alone were included in the study. The incidence of micronuclei (MN) was evaluated to determine the induction of AR. Results: The results indicated that the cells which were exposed to AD of RF in G 0- and G1-phase of the cell cycle did not exhibit AR while such a response was observed when the cells were exposed to AD of RF in S-phase of the cell cycle. Conclusions: These results confirmed the observations reported in our previous investigation where AR was observed in human blood lymphocytes exposed to AD of RF in S-phase of the cell cycle and further suggested that the timing of AD exposure of RF is important to elicit AR.
KW - Adaptive response
KW - Cell cycle
KW - Human blood lymphocytes
KW - Micronuclei
KW - Radiofrequency fields
UR - http://www.scopus.com/inward/record.url?scp=80054787567&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80054787567&partnerID=8YFLogxK
U2 - 10.3109/09553002.2011.574779
DO - 10.3109/09553002.2011.574779
M3 - Article
C2 - 21557704
AN - SCOPUS:80054787567
VL - 87
SP - 993
EP - 999
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
SN - 0955-3002
IS - 9
ER -