Inducible (Type II) nitric oxide synthase in human placental villous tissue of normotensive, pre-eclamptic and intrauterine growth-restricted pregnancies

L. Myatt, A. L W Eis, D. E. Brockman, W. Kossenjans, I. Greer, F. Lyall

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We have utilized two distinct monospecific antibodies against the type II (macrophage or inducible) nitric oxide synthase (NOS) isoform to localize the distribution of the enzyme within the human placenta in tissues from normotensive pregnancies and those complicated by pre-eclampsia and/or intrauterine growth restriction. Both antibodies immunolocalize to cells in the villous stroma on frozen sections of villous tissue. Colocalization studies with anti-CD14 or anti-CD45 antibodies that recognize cells of leucocyte or monocyte/macrophage lineage indicate that Hofbauer cells are expressing type II NOS. This is in contrast to expression of type III (endothelial) NOS which is seen in syncytiotrophoblast and in villous vascular endothelium. In some, but not all, normotensive and pathologic placental tissue, some type II NOS immunostaining could be seen in syncytiotrophoblast and vascular endothelium; however, no differences could be discerned between groups of tissues. Expression of type II NOS by Hofbauer cells may indicate they are involved in surveillance against maternal immune insult or maternal pathogens whereby they secrete nitric oxide to exert a cytostatic/cytotoxic effect.

Original languageEnglish (US)
Pages (from-to)261-268
Number of pages8
JournalPlacenta
Volume18
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

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Nitric Oxide Synthase Type II
Pregnancy
Vascular Endothelium
Trophoblasts
Growth
Macrophages
Mothers
Antibodies
Nitric Oxide Synthase Type III
Frozen Sections
Cytostatic Agents
Pre-Eclampsia
Placenta
Monocytes
Anti-Idiotypic Antibodies
Nitric Oxide
Protein Isoforms
Leukocytes
Enzymes

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Inducible (Type II) nitric oxide synthase in human placental villous tissue of normotensive, pre-eclamptic and intrauterine growth-restricted pregnancies. / Myatt, L.; Eis, A. L W; Brockman, D. E.; Kossenjans, W.; Greer, I.; Lyall, F.

In: Placenta, Vol. 18, No. 4, 1997, p. 261-268.

Research output: Contribution to journalArticle

Myatt, L. ; Eis, A. L W ; Brockman, D. E. ; Kossenjans, W. ; Greer, I. ; Lyall, F. / Inducible (Type II) nitric oxide synthase in human placental villous tissue of normotensive, pre-eclamptic and intrauterine growth-restricted pregnancies. In: Placenta. 1997 ; Vol. 18, No. 4. pp. 261-268.
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