Increased placental XIAP and caspase 3 is associated with increased placental apoptosis in a baboon model of maternal nutrient reduction

Juan A. Arroyo, Cun Li, Natalia Schlabritz-Loutsevitch, Tom McDonald, Peter Nathanielsz, Henry L. Galan

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Objective: Our objective was to determine signaling molecules and apoptosis rate in the term placenta of a baboon model of maternal nutrient reduction (MNR). Study Design: Female baboons were fed ad libitum for controls (n = 7) or 70% of control baboon diet (MNR; n = 6) from 30-165 days of gestation with necropsy at 165 days of gestation. Placental tissues were collected and fixed for immunohistochemistry or snap frozen to measure extracellular signal-regulated kinases, protein kinase B, JUN NH2-terminal kinase, X-linked inhibitor of apoptosis protein, and caspase 3. Placental villous apoptosis was determined by terminal deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′-triphosphate nick-end labeling and cytokeratin 18 cleavage. Results: Compared with the control placentas, MNR placentas demonstrated reduced placental weight (P < .02), decreased phospho (p)-ERK (P < .04), increased placental villous apoptosis (P < .001), increased villous cytokeratin 18 cleavage, increased X-linked inhibitor of apoptosis protein (P < .007), and increased active caspase 3 (P < .02). Conclusion: We conclude that placental apoptosis is increased in this baboon model of MNR at term and that the increase in X-linked inhibitor of apoptosis protein may be a protective mechanism against this apoptosis.

Original languageEnglish (US)
Pages (from-to)364.e13-364.e18
JournalAmerican Journal of Obstetrics and Gynecology
Volume203
Issue number4
DOIs
StatePublished - Oct 2010

Keywords

  • apoptosis
  • caspase 3
  • MNR
  • placenta
  • XIAP

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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