Increased fatigability of masseter muscles with age: correlation with enzyme activities in male and female fischer 344 rats

R. McCarter, Z. Tai, M. Norton

    Research output: Contribution to journalArticle

    Abstract

    We previously demonstrated increased fatigability in vitro of masseter muscles from male and female rats with advancing age. The goal of the present experiments was to determine if this loss of endurance is associated with decreased activity of enzymes involved in energy metabolism. Male and female Fischer 344 rats were obtained from the Harlan-NIA colony at ages 6, 12, 18 and 24 months. Masseter muscle enzyme activities were measured at 30°C using muscle homogenates prepared in 175 mM KCl containing 10 mM Tris-HCl and 2 mM EDTA at pH 7.2. Cytochrome oxidase, citrate synthase and succinate dehydrogenase levels served as indices oxidative capacity. Lactate dehydrogenase and phosphorylase activities were used to assess glycolyuc potential. Carnitine palmitoyl-transferase and 3-hydroxyacyl CoA dehydrogenase activities were used to assess capacity for fatty acid oxidation. Only citrate synthase activity correlated with the observed decrease in endurance of muscles from male and female rats. We conclude that the supply of energy in fatiguing contractions of masseter muscles of elderly rats may be rate-limited by citrate synthase enzyme activity.

    Original languageEnglish (US)
    JournalFASEB Journal
    Volume11
    Issue number3
    StatePublished - 1997

    Fingerprint

    Masseter Muscle
    Citrate (si)-Synthase
    Inbred F344 Rats
    Enzyme activity
    citrate (si)-synthase
    Muscle
    Rats
    enzyme activity
    muscles
    rats
    Enzymes
    3-Hydroxyacyl-CoA Dehydrogenase
    Muscles
    Phosphorylases
    Succinate Dehydrogenase
    Carnitine
    Electron Transport Complex IV
    Transferases
    3-Hydroxyacyl CoA Dehydrogenases
    carnitine palmitoyltransferase

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Cell Biology

    Cite this

    Increased fatigability of masseter muscles with age : correlation with enzyme activities in male and female fischer 344 rats. / McCarter, R.; Tai, Z.; Norton, M.

    In: FASEB Journal, Vol. 11, No. 3, 1997.

    Research output: Contribution to journalArticle

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