Increased expression of macrophage colony-stimulating factor and its receptor in patients with endometriosis

Nicole M. Budrys, Hareesh B. Nair, Ya-guang Liu, Nameer B Kirma, Peter A. Binkley, Shantha Kumar, Robert S Schenken, Rajeshwar R Tekmal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis. Design: In vivo and vitro study. Setting: University-based academic medical center. Patient(s): Reproductive-age women undergoing surgery for benign conditions. Intervention(s): Peritoneal and endometrial tissue samples were obtained. Main Outcome Measure(s): CSF-1 and C-FMS expression. Result(s): Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis. Conclusion(s): Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.

Original languageEnglish (US)
JournalFertility and Sterility
Volume97
Issue number5
DOIs
StatePublished - May 2012

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Macrophage Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Endometriosis
Coculture Techniques
Choristoma
Ascitic Fluid
Epithelial Cells

Keywords

  • C-FMS
  • cell culture
  • colony-stimulating factor 1
  • early lesion development
  • Endometriosis
  • macrophage
  • peritoneal fluid

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Increased expression of macrophage colony-stimulating factor and its receptor in patients with endometriosis. / Budrys, Nicole M.; Nair, Hareesh B.; Liu, Ya-guang; Kirma, Nameer B; Binkley, Peter A.; Kumar, Shantha; Schenken, Robert S; Tekmal, Rajeshwar R.

In: Fertility and Sterility, Vol. 97, No. 5, 05.2012.

Research output: Contribution to journalArticle

Budrys, Nicole M. ; Nair, Hareesh B. ; Liu, Ya-guang ; Kirma, Nameer B ; Binkley, Peter A. ; Kumar, Shantha ; Schenken, Robert S ; Tekmal, Rajeshwar R. / Increased expression of macrophage colony-stimulating factor and its receptor in patients with endometriosis. In: Fertility and Sterility. 2012 ; Vol. 97, No. 5.
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AU - Schenken, Robert S

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