Increased expression of macrophage colony-stimulating factor and its receptor in patients with endometriosis

Nicole M. Budrys, Hareesh B. Nair, Ya Guang Liu, Nameer B. Kirma, Peter A. Binkley, Shantha Kumar, Robert S. Schenken, Rajeshwar Rao Tekmal

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Objective: To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis. Design: In vivo and vitro study. Setting: University-based academic medical center. Patient(s): Reproductive-age women undergoing surgery for benign conditions. Intervention(s): Peritoneal and endometrial tissue samples were obtained. Main Outcome Measure(s): CSF-1 and C-FMS expression. Result(s): Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis. Conclusion(s): Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.

Original languageEnglish (US)
Pages (from-to)1129-1135.e1
JournalFertility and sterility
Volume97
Issue number5
DOIs
StatePublished - May 2012

Keywords

  • C-FMS
  • Endometriosis
  • cell culture
  • colony-stimulating factor 1
  • early lesion development
  • macrophage
  • peritoneal fluid

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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