Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity

H. E.J. Yong, P. Murthi, A. Borg, B. Kalionis, E. K. Moses, S. P. Brennecke, R. J. Keogh

    Research output: Contribution to journalArticlepeer-review

    16 Scopus citations

    Abstract

    Introduction Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1, INHA, INHBB, ERAP1, ERAP2, LNPEP, COL4A1 and COL4A2. The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods Third trimester decidual tissues were collected from both normotensive (n = 21) and SPE pregnancies (n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann-Whitney U test and Spearman's Correlation. Results The data demonstrate significantly increased decidual mRNA expression levels of ACVR1, INHBB, ERAP1, ERAP2, LNPEP, COL4A1 and COL4A2 in SPE (p < 0.05). Increased mRNA expression levels of several genes - INHA, INHBB, COL4A1 and COL4A2 were correlated with earlier onset of PE and earlier delivery of the fetus (p < 0.05). Conclusion These results suggest altered expression of maternal susceptibility genes may play roles in PE development and the course of disease severity.

    Original languageEnglish (US)
    Pages (from-to)117-124
    Number of pages8
    JournalPlacenta
    Volume35
    Issue number2
    DOIs
    StatePublished - Feb 2014

    Keywords

    • Clinical severity
    • Decidua
    • Gene expression
    • Pre-eclampsia
    • Susceptibility genes

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology
    • Developmental Biology

    Fingerprint Dive into the research topics of 'Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity'. Together they form a unique fingerprint.

    Cite this