Increased calcium bioavailability in mice fed genetically engineered plants lacking calcium oxalate

Jay Morris, Paul A. Nakata, Michele McConn, Amanda Brock, Kendal D. Hirschi

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Bioavailable calcium affects bone formation and calcification. Here we investigate how a single gene mutation altering calcium partitioning in the model forage crop Medicago truncatula affects calcium bioavailability. Previously, the cod5 M. truncatula mutant was identified which contains identical calcium concentrations to wild-type, but contains no oxalate crystals. In this study, equal number of male and female mice were randomly grouped and then fed one of four 45Ca-containing diets: M. truncatula extrinsically or intrinsically labeled, and cod5 extrinsically or intrinsically labeled. Absorption of the tracer was determined in the legs one day after consumption. The absorption was similar in the M. truncatula and cod5 extrinsically labeled diets; however, in the intrinsically labeled diets, calcium absorption was 22.87% (P < 0.001) higher in mice fed cod5. Our study presents the first genetic evidence demonstrating the nutritional impact of removing oxalate crystals from foods.

Original languageEnglish (US)
Pages (from-to)613-618
Number of pages6
JournalPlant Molecular Biology
Issue number5
StatePublished - Jul 2007
Externally publishedYes


  • Calcium bioavailability
  • Medicago truncatula
  • Mice
  • Oxalate crystal

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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