Increased Adipocyte Area in Injured Muscle With Aging and Impaired Remodeling in Female Mice

Caitlin M. Fearing, David W. Melton, Xiufen Lei, Heather Hancock, Hanzhou Wang, Zaheer U. Sarwar, Laurel Porter, Matthew McHale, Linda M Mcmanus, Paula K Shireman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We demonstrated that young male and female mice similarly regenerated injured skeletal muscle; however, female mice transiently increased adipocyte area within regenerated muscle in a sex hormone-dependent manner. We extended these observations to investigate the effect of aging and sex on sarcopenia and muscle regeneration. Cardiotoxin injury to the tibialis anterior muscle of young, middle, and old-aged C57Bl/6J male and female mice was used to measure regenerated myofiber cross-sectional area (CSA), adipocyte area, residual necrosis, and inflammatory cell recruitment. Baseline (uninjured) myofiber CSA was decreased in old mice of both sexes compared to young and middle-aged mice. Regenerated CSA was similar in male mice in all age groups until baseline CSA was attained but decreased in middle and old age female mice compared to young females. Furthermore, adipocyte area within regenerated muscle was transiently increased in young females compared to young males and these sex-dependent increases persisted in middle and old age female mice and were associated with increased Pparg. Young female mice had more pro-inflammatory monocytes/macrophages in regenerating muscle than young male mice and increased Sca-1+CD45-cells. In conclusion, sex and age influence pro-inflammatory cell recruitment, muscle regeneration, and adipocyte area following skeletal muscle injury.

Original languageEnglish (US)
Pages (from-to)992-1004
Number of pages13
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume71
Issue number8
DOIs
StatePublished - Aug 1 2016

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Adipocytes
Muscles
Regeneration
Skeletal Muscle
Cardiotoxins
Sarcopenia
Wounds and Injuries
Gonadal Steroid Hormones
Muscle Cells
Monocytes
Necrosis
Age Groups
Macrophages

Keywords

  • Monocyte/macrophage
  • Muscle regeneration
  • Sarcopenia

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Increased Adipocyte Area in Injured Muscle With Aging and Impaired Remodeling in Female Mice. / Fearing, Caitlin M.; Melton, David W.; Lei, Xiufen; Hancock, Heather; Wang, Hanzhou; Sarwar, Zaheer U.; Porter, Laurel; McHale, Matthew; Mcmanus, Linda M; Shireman, Paula K.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 71, No. 8, 01.08.2016, p. 992-1004.

Research output: Contribution to journalArticle

Fearing, Caitlin M. ; Melton, David W. ; Lei, Xiufen ; Hancock, Heather ; Wang, Hanzhou ; Sarwar, Zaheer U. ; Porter, Laurel ; McHale, Matthew ; Mcmanus, Linda M ; Shireman, Paula K. / Increased Adipocyte Area in Injured Muscle With Aging and Impaired Remodeling in Female Mice. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2016 ; Vol. 71, No. 8. pp. 992-1004.
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