Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA

Yu Liu, Erik Holmstrom, Ping Yu, Kemin Tan, Xiaobing Zuo, David J. Nesbitt, Rui J Sousa, Jason R. Stagno, Yun Xing Wang

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Site-specific incorporation of labeled nucleotides is an extremely useful synthetic tool for many structural studies (e.g., NMR, electron paramagnetic resonance (EPR), fluorescence resonance energy transfer (FRET), and X-ray crystallography) of RNA. However, specific-position-labeled RNAs >60 nt are not commercially available on a milligram scale. Position-selective labeling of RNA (PLOR) has been applied to prepare large RNAs labeled at desired positions, and all the required reagents are commercially available. Here, we present a step-by-step protocol for the solid-liquid hybrid phase method PLOR to synthesize 71-nt RNA samples with three different modification applications, containing (i) a 13C 15 N-labeled segment; (ii) discrete residues modified with Cy3, Cy5, or biotin; or (iii) two iodo-U residues. The flexible procedure enables a wide range of downstream biophysical analyses using precisely localized functionalized nucleotides. All three RNAs were obtained in <2 d, excluding time for preparing reagents and optimizing experimental conditions. With optimization, the protocol can be applied to other RNAs with various labeling schemes, such as ligation of segmentally labeled fragments.

Original languageEnglish (US)
Pages (from-to)987-1005
Number of pages19
JournalNature Protocols
Volume13
Issue number5
DOIs
StatePublished - May 1 2018

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Liu, Y., Holmstrom, E., Yu, P., Tan, K., Zuo, X., Nesbitt, D. J., Sousa, R. J., Stagno, J. R., & Wang, Y. X. (2018). Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA. Nature Protocols, 13(5), 987-1005. https://doi.org/10.1038/nprot.2018.002