In Vivo Whole Animal Body Imaging Reveals Colonization of Chlamydia muridarum to the Lower Genital Tract at Early Stages of Infection

Rishein Gupta, Shradha Wali, Jieh Juen Yu, James P. Chambers, Guangming Zhong, Ashlesh K. Murthy, Sazaly Abu Bakar, M. N. Guentzel, Bernard P. Arulanandam

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Purpose: The leading cause of sexually transmitted bacterial infection is Chlamydia trachomatis. The aim of this study is to investigate the early events in colonization of this bacterium within the murine genital tract.

Procedures: An in vivo animal body imaging technology was used to track fluorophore labeled C. muridarum elementary bodies (EBs) inoculated intravaginally in C57BL/6 mice during the first 24 h of infection.

Results: Ascension of viable EBs was observed (1) to be localized to the lower regions of the murine genital tract within the first 24 h post challenge and (2) was dose independent during this early exposure period. Molecular detection revealed enhanced bacterial load in lower regions of the genital tract with increasing bacterial load in the upper region beginning 12 h post inoculation.

Conclusion: This study provides additional insight into chlamydial colonization in the murine genital tract during the first 12–24 h following inoculation.

Original languageEnglish (US)
Pages (from-to)635-641
Number of pages7
JournalMolecular Imaging and Biology
Volume16
Issue number5
DOIs
StatePublished - 2014

Keywords

  • Bacterial ascension
  • Chlamydia muridarum
  • Genital infections
  • Host immunity
  • In vivo imaging

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

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