In vivo regulation of matrix vesicle concentration and enzyme activity during primary bone formation

Z. Schwartz, L. Swain, J. Sela, U. Gross, D. Amir, D. Kohavi, C. Muller-Mai, B. Boyan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

In vivo regulation of matrix vesicles (MV) during primary bone formation was examined using tibial marrow ablation in rats as the experimental model. The effects of bone-bonding and nonbonding implants on the number of MV/μm2 of matrix and the alkaline phosphatase (ALPase) and phospholipase A2 (PA2) activities of MV-enriched microsomes (MVEM) isolated from the healing bone were studied. MV concentration, ALPase, and PA2 were increased by bone-bonding implants by day 3 post-surgery; a similar effect was seen in the contralateral limb, but at a lower magnitude. Nonbonding implants had no effect at day 3 and decreased MV concentration and PA2 activity at later time points; the same behavior was observed in the contralateral limb. These results demonstrate that MVs are influenced in a differential manner by implant materials, both locally and systemically, and can be regulated during primary mineralization.

Original languageEnglish (US)
Pages (from-to)134-138
Number of pages5
JournalBone and Mineral
Volume17
Issue number2
DOIs
StatePublished - May 1992

ASJC Scopus subject areas

  • Surgery
  • Biochemistry
  • Endocrinology

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    Schwartz, Z., Swain, L., Sela, J., Gross, U., Amir, D., Kohavi, D., Muller-Mai, C., & Boyan, B. (1992). In vivo regulation of matrix vesicle concentration and enzyme activity during primary bone formation. Bone and Mineral, 17(2), 134-138. https://doi.org/10.1016/0169-6009(92)90724-R