@article{07066017968e4b64afd56d82d6325f4a,
title = "In Vivo Imaging of Tumor-Propagating Cells, Regional Tumor Heterogeneity, and Dynamic Cell Movements in Embryonal Rhabdomyosarcoma",
abstract = "Embryonal rhabdomyosarcoma (ERMS) is an aggressive pediatric sarcoma of muscle. Here, we show that ERMS-propagating potential is confined to myf5+ cells and can be visualized in live, fluorescent transgenic zebrafish. During early tumor growth, myf5+ ERMS cells reside adjacent normal muscle fibers. By late-stage ERMS, myf5+ cells are reorganized into distinct regions separated from differentiated tumor cells. Time-lapse imaging of late-stage ERMS revealed that myf5+ cells populate newly formed tumor only after seeding by highly migratory myogenin+ ERMS cells. Moreover, myogenin+ ERMS cells can enter the vasculature, whereas myf5+ ERMS-propagating cells do not. Our data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.",
author = "Ignatius, {Myron S.} and Eleanor Chen and Elpek, {Natalie M.} and Fuller, {Adam Z.} and Tenente, {In{\^e}s M.} and Ryan Clagg and Sali Liu and Blackburn, {Jessica S.} and Linardic, {Corinne M.} and Rosenberg, {Andrew E.} and Nielsen, {Petur G.} and Mempel, {Thorsten R.} and Langenau, {David M.}",
note = "Funding Information: E.C. and J.S.B. are supported by the National Institutes of Health (NIH) Training Grants T32 HL007627 and 5T32CA09216-26, respectively. C.M.L. is supported by R01 CA122706 and K12 HD043494. D.M.L. is supported by NIH Grants K01 AR055619, 1RO1CA154923, and 1R21CA156056; the Alex's Lemonade Stand Foundation; the Sarcoma Foundation of America; the American Cancer Society; and the Harvard Stem Cell Institute. I.M.T. is supported by Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (the Portuguese Foundation for Science and Technology) through Fellowship SFRH/BD/51288/2010. We thank Huai-Jen Tsai for myf5-GFP transgenic animals and Clarrisa Henry for critical review of our manuscript. ",
year = "2012",
month = may,
day = "25",
doi = "10.1016/j.ccr.2012.03.043",
language = "English (US)",
volume = "21",
pages = "680--693",
journal = "Cancer Cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "5",
}