In vivo hepatic oxidative stress because of carbon tetrachloride toxicity

Protection by melatonin and pinoline

M. Aranda, C. D. Albendea, F. Lostalé, L. López-Pingarrón, L. Fuentes-Broto, E. Martínez-Ballarín, Russel J Reiter, M. C. Pérez-Castejón, J. J. García

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The protective in vivo effects of melatonin or pinoline on carbon tetrachloride (CCl4)-induced oxidative damage were investigated in liver of rats and compared to rats injected only with CCl4 (5 mL/kg body weight). Hepatic cell membrane fluidity, monitored using fluorescence spectroscopy, exhibited a significant decrease in animals exposed to CCl 4 compared to control rats. Increases in lipid and protein oxidation, as assessed by concentrations of malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA), and protein carbonylation, respectively, were also seen in hepatic homogenates of animals exposed to CCl4. The administration of melatonin (10 mg/kg body weight) or pinoline injected 30 min before and 1 hr after CCl4, fully prevented membrane rigidity and protein oxidation. However, treatment with melatonin was more effective in terms of reducing lipid peroxidation than pinoline, as the increases in MDA+4-HDA levels because of CCl4 were reduced by 93.4% and 34.4% for melatonin or pinoline, respectively. Livers from CCl4-injected rats showed several histopathological alterations; above all, there were signs of necrosis and ballooning degeneration. The concurrent administration of melatonin or pinoline reduced the severity of these morphological changes. On the basis of the biochemical and histopathological findings, we conclude that both melatonin and pinoline were highly effective in protecting the liver against oxidative damage and membrane rigidity because of CCl4. Therefore, these indoles may be useful as cotreatments for patients with hepatic intoxication induced by CCl4.

Original languageEnglish (US)
Pages (from-to)78-85
Number of pages8
JournalJournal of Pineal Research
Volume49
Issue number1
DOIs
StatePublished - 2010

Fingerprint

Carbon Tetrachloride
Melatonin
Oxidative Stress
Liver
Malondialdehyde
Body Weight
Protein Carbonylation
Indoles
Membrane Fluidity
Fluorescence Spectrometry
Lipid Peroxidation
6-methoxytryptoline
Hepatocytes
Membrane Proteins
Necrosis
Cell Membrane
Lipids
Membranes
Proteins

Keywords

  • carbon tetrachloride
  • melatonin
  • membrane fluidity
  • oxidative stress
  • pinoline

ASJC Scopus subject areas

  • Endocrinology
  • Medicine(all)

Cite this

Aranda, M., Albendea, C. D., Lostalé, F., López-Pingarrón, L., Fuentes-Broto, L., Martínez-Ballarín, E., ... García, J. J. (2010). In vivo hepatic oxidative stress because of carbon tetrachloride toxicity: Protection by melatonin and pinoline. Journal of Pineal Research, 49(1), 78-85. https://doi.org/10.1111/j.1600-079X.2010.00769.x

In vivo hepatic oxidative stress because of carbon tetrachloride toxicity : Protection by melatonin and pinoline. / Aranda, M.; Albendea, C. D.; Lostalé, F.; López-Pingarrón, L.; Fuentes-Broto, L.; Martínez-Ballarín, E.; Reiter, Russel J; Pérez-Castejón, M. C.; García, J. J.

In: Journal of Pineal Research, Vol. 49, No. 1, 2010, p. 78-85.

Research output: Contribution to journalArticle

Aranda, M, Albendea, CD, Lostalé, F, López-Pingarrón, L, Fuentes-Broto, L, Martínez-Ballarín, E, Reiter, RJ, Pérez-Castejón, MC & García, JJ 2010, 'In vivo hepatic oxidative stress because of carbon tetrachloride toxicity: Protection by melatonin and pinoline', Journal of Pineal Research, vol. 49, no. 1, pp. 78-85. https://doi.org/10.1111/j.1600-079X.2010.00769.x
Aranda M, Albendea CD, Lostalé F, López-Pingarrón L, Fuentes-Broto L, Martínez-Ballarín E et al. In vivo hepatic oxidative stress because of carbon tetrachloride toxicity: Protection by melatonin and pinoline. Journal of Pineal Research. 2010;49(1):78-85. https://doi.org/10.1111/j.1600-079X.2010.00769.x
Aranda, M. ; Albendea, C. D. ; Lostalé, F. ; López-Pingarrón, L. ; Fuentes-Broto, L. ; Martínez-Ballarín, E. ; Reiter, Russel J ; Pérez-Castejón, M. C. ; García, J. J. / In vivo hepatic oxidative stress because of carbon tetrachloride toxicity : Protection by melatonin and pinoline. In: Journal of Pineal Research. 2010 ; Vol. 49, No. 1. pp. 78-85.
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