In vivo generation of lung and thyroid tissues from embryonic stem cells using blastocyst complementation

Bingqiang Wen, Enhong Li, Vladimir Ustiyan, Guolun Wang, Minzhe Guo, Cheng Lun Na, Gregory T. Kalin, Veronica Galvan, Yan Xu, Timothy E. Weaver, Tanya V. Kalin, Jeffrey A. Whitsett, Vladimir V. Kalinichenko

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Rationale: The regeneration and replacement of lung cells or tissues from induced pluripotent stem cell– or embryonic stem cell–derived cells represent future therapies for life-threatening pulmonary disorders but are limited by technical challenges to produce highly differentiated cells able to maintain lung function. Functional lung tissue–containing airways, alveoli, vasculature, and stroma have never been produced via directed differentiation of embryonic stem cells (ESCs) or induced pluripotent stem cells. We sought to produce all tissue components of the lung from bronchi to alveoli by embryo complementation. Objectives: To determine whether ESCs are capable of generating lung tissue in Nkx2-12/2 mouse embryos with lung agenesis. Methods: Blastocyst complementation was used to produce chimeras from normal mouse ESCs and Nkx2-12/2 embryos, which lack pulmonary tissues. Nkx2-12/2 chimeras were examined using immunostaining, transmission electronic microscopy, fluorescence-activated cell sorter analysis, and single-cell RNA sequencing. Measurements and Main Results: Although peripheral pulmonary and thyroid tissues are entirely lacking in Nkx2-1 gene-deleted embryos, pulmonary and thyroid structures in Nkx2-12/2 chimeras were restored after ESC complementation. Respiratory epithelial cell lineages in restored lungs of Nkx2-12/2 chimeras were derived almost entirely from ESCs, whereas endothelial, immune, and stromal cells were mosaic. ESC-derived cells from multiple respiratory cell lineages were highly differentiated and indistinguishable from endogenous cells based on morphology, ultrastructure, gene expression signatures, and cell surface proteins used to identify cell types by fluorescence-activated cell sorter. Conclusions: Lung and thyroid tissues were generated in vivo from ESCs by blastocyst complementation. Nkx2-12/2 chimeras can be used as “bioreactors” for in vivo differentiation and functional studies of ESC-derived progenitor cells.

Original languageEnglish (US)
Pages (from-to)471-483
Number of pages13
JournalAmerican Journal of Respiratory and Critical Care Medicine
Issue number4
StatePublished - Feb 15 2021


  • Blastocyst complementation
  • Generation of lung tissue from embryonic stem cells
  • Lung development
  • Lung regenerative medicine for pediatric lung diseases
  • Nkx2-1 transcription factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


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