In vivo depth-resolved oxygen saturation by dual-wavelength photothermal (DWP) OCT

Roman V. Kuranov, Shams Kazmi, Austin B. McElroy, Jeffrey W. Kiel, Andrew K. Dunn, Thomas E. Milner, Timothy Q. Duong

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Microvasculature hemoglobin oxygen saturation (SaO2) is important in the progression of various pathologies. Non-invasive depthresolved measurement of SaO2 levels in tissue microvasculature has the potential to provide early biomarkers and a better understanding of the pathophysiological processes allowing improved diagnostics and prediction of disease progression. We report proof-of-concept in vivo depth-resolved measurement of SaO2 levels in selected 30 μm diameter arterioles in the murine brain using Dual-Wavelength Photothermal (DWP) Optical Coherence Tomography (OCT) with 800 nm and 770 nm photothermal excitation wavelengths. Depth location of back-reflected light from a target arteriole was confirmed using Doppler and speckle contrast OCT images. SaO2 measured in a murine arteriole with DWP-OCT is linearly correlated (R2=0.98) with systemic SaO2 values recorded by a pulse-oximeter. DWP-OCT are steadily lower (10.1%) than systemic SaO2 values except during pure oxygen breathing. DWP-OCT is insensitive to OCT intensity variations and is a candidate approach for in vivo depth-resolved quantitative imaging of microvascular SaO2 levels.

Original languageEnglish (US)
Pages (from-to)23831-23844
Number of pages14
JournalOptics Express
Volume19
Issue number24
DOIs
StatePublished - Nov 21 2011

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Kuranov, R. V., Kazmi, S., McElroy, A. B., Kiel, J. W., Dunn, A. K., Milner, T. E., & Duong, T. Q. (2011). In vivo depth-resolved oxygen saturation by dual-wavelength photothermal (DWP) OCT. Optics Express, 19(24), 23831-23844. https://doi.org/10.1364/OE.19.023831