In vivo calcium imaging visualizes incision-induced primary afferent sensitization and its amelioration by capsaicin pretreatment

Hirotake Ishida, Yan Zhang, Ruben Gomez, John Shannonhouse, Hyeonwi Son, Ratan Banik, Yu Shin Kim

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Previous studies have shown that infiltration of capsaicin into the surgical site can prevent incision-induced spontaneous pain like behaviors and heat hyperalgesia. In the present study, we aimed to monitor primary sensory neuron Ca2+ activity in the intact dorsal root ganglia (DRG) using Pirt-GCaMP3 male and female mice pretreated with capsaicin or vehicle before the plantar incision. Intraplantar injection of capsaicin (0.05%) significantly attenuated spontaneous pain, mechanical, and heat hypersensitivity after plantar incision. The Ca2+ response in in vivo DRG and in in situ spinal cord was significantly enhanced in the ipsilateral side compared with contralateral side or naive control. Primary sensory nerve fiber length was significantly decreased in the incision skin area in capsaicin-pretreated animals detected by immunohistochemistry and placental alkaline phosphatase (PLAP) staining. Thus, capsaicin pretreatment attenuates incisional pain by suppressing Ca2+ response because of degeneration of primary sensory nerve fibers in the skin.

Original languageEnglish (US)
Pages (from-to)8494-8507
Number of pages14
JournalJournal of Neuroscience
Volume41
Issue number41
DOIs
StatePublished - Oct 13 2021

Keywords

  • Calcium imaging
  • In vivo
  • Postoperative pain

ASJC Scopus subject areas

  • Neuroscience(all)

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