In vivo assessment of genotoxic effects of Annona squamosa seed extract in rats

Paramjit Grover, S. P. Singh, P. V. Prabhakar, Utkarsh A. Reddy, A. Balasubramanyam, M. Mahboob, M. F. Rahman, Sunil Misra

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Widespread use of pesticides represents a potential risk to human and environmental health. Hence, biopesticides from plants are some of the future strategies for plant protection. In this regard, a seed extract of Annona squamosa was prepared and found to be a promising pesticide. In order to establish the inherent toxicity and non-target safety required for registration and marketing of pesticides, toxicological studies are conducted. The genotoxicity potential was evaluated in rats with 75, 150 and 300 mg/kg Annona squamosa by the comet assay in leucocytes, micronucleus and chromosomal aberration tests in bone marrow. We also studied the effects of 300 mg/kg of extract on lipid peroxidation, reduced glutathione level and glutathione S transferase activity in liver, lungs, brain, kidneys, heart and spleen of treated rats. The comet assay showed a statistically significant dose related increase in DNA migration. The micronucleus and chromosomal aberration tests revealed a significant induction in frequency of micronuclei and chromosomal aberrations at 150 and 300 mg/kg. Annona squamosa treatment significantly enhanced lipid peroxidation, decreased glutathione and glutathione S transferase levels revealing the oxidative stress condition. Our results warrant careful use of Annona squamosa seed extract as a biopesticide till more tests are carried out.

Original languageEnglish (US)
Pages (from-to)1964-1971
Number of pages8
JournalFood and Chemical Toxicology
Volume47
Issue number8
DOIs
StatePublished - Aug 2009
Externally publishedYes

Keywords

  • Annona squamosa
  • Antioxidants
  • Genotoxicity
  • Lipid peroxidation
  • Rat
  • Seed extract

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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