In vivo and in vitro effects of the pineal gland and melatonin on [Ca2++ Mg2+]‐dependent ATPase in cardiac sarcolemma

Li‐Dun ‐D Chen, Dun‐Xian ‐X Tan, Russel J. Reiter, Ken Yaga, Burkhard Poeggeler, Pramod Kumar, Lucien C. Manchester, James P. Chambers

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Abstract: The possible diurnal variation in cardiac [Ca2++ Mg2+]‐dependent ATPase (Ca2+ pump) activity and the influence of pinealectomy and melatonin on this enzyme in rat heart have been studied. Lowest levels of cardiac sarcolemma] membrane [Ca2++ Mg2+]‐dependent ATPase activity were measured in late afternoon in rats kept under a 14:10 light:dark cycle. Late in the dark phase the enzyme activity began to increase with the rise continuing until 0900, 3 hr after light onset. These time‐dependent changes in [Ca2++ Mg2+]‐dependent ATPase activity did not occur in either pinealectomized or light‐exposed rats suggesting that melatonin, secreted from the pineal gland during the night, induces the change in [Ca2++ Mg2+]‐dependent ATPase activity. In vitro studies in which cardiac tissue was incubated in the presence of melatonin over a wide range of doses showed that this indole stimulated the Ca2+ pump. The half‐maximal effect of melatonin was observed at a melatonin concentration of 28 ng/ml. These findings suggest that the daily change in [Ca2++ Mg2+]‐dependent ATPase activity in the sarcolemma of heart tissue is a result of the circadian rhythm in pineal melatonin production and secretion. These findings may be applicable to normal cardiac physiology.

Original languageEnglish (US)
Pages (from-to)178-183
Number of pages6
JournalJournal of pineal research
Volume14
Issue number4
DOIs
StatePublished - May 1993

Keywords

  • [Ca+ Mg]‐dependent ATPase
  • heart
  • melatonin
  • pineal gland
  • sarcolemma

ASJC Scopus subject areas

  • Endocrinology

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