TY - JOUR
T1 - In vitro susceptibilities of cerebrospinal fluid isolates of Cryptococcus neoformans collected during a ten-year period against fluconazole, voriconazole and posaconazole (SCH56592)
AU - Yildiran, S. T.
AU - Fothergill, A. W.
AU - Sutton, D. A.
AU - Rinaldi, M. G.
PY - 2002/11/30
Y1 - 2002/11/30
N2 - We investigated the in vitro susceptibilities of 213 cerebrospinal fluid isolates of Cryptococcus neoformans isolated from 192 patients through a 10-year period, 1990-99, against fluconazole, voriconazole and posaconazole (SCH56592) by using the NCCLS (National Committee for Clinical Laboratory Standards) macrodilution method, M27-A. The overall MICs50 and MICs90 of fluconazole, voriconazole and posaconazole were found to be 2 and 8 μg ml-1, ≤ 0.125 μg ml-1 (both), and ≤ 0.015 and 0.06 μg ml-1, respectively. The MIC ranges, MICs50, and MICs90 of three triazoles were also determined according to the defined year category (1990-94, 1995, 1996, 1997 and 1998-99). The MICs50 and MICs90 of each triazole remained almost unchanged and did not exhibit any sign of an upward shift during a decade (1990-99). However, a tendency for development of possible cross-resistance between the three triazoles was observed only in 1996. Meanwhile, the individual relapsing isolates (n:21) from 20 patients exhibited same, higher or, suprisingly, lower MICs, particularly against fluconazole and posaconazole.
AB - We investigated the in vitro susceptibilities of 213 cerebrospinal fluid isolates of Cryptococcus neoformans isolated from 192 patients through a 10-year period, 1990-99, against fluconazole, voriconazole and posaconazole (SCH56592) by using the NCCLS (National Committee for Clinical Laboratory Standards) macrodilution method, M27-A. The overall MICs50 and MICs90 of fluconazole, voriconazole and posaconazole were found to be 2 and 8 μg ml-1, ≤ 0.125 μg ml-1 (both), and ≤ 0.015 and 0.06 μg ml-1, respectively. The MIC ranges, MICs50, and MICs90 of three triazoles were also determined according to the defined year category (1990-94, 1995, 1996, 1997 and 1998-99). The MICs50 and MICs90 of each triazole remained almost unchanged and did not exhibit any sign of an upward shift during a decade (1990-99). However, a tendency for development of possible cross-resistance between the three triazoles was observed only in 1996. Meanwhile, the individual relapsing isolates (n:21) from 20 patients exhibited same, higher or, suprisingly, lower MICs, particularly against fluconazole and posaconazole.
KW - Antifungal susceptibility
KW - Cerebrospinal fluid
KW - Cryptococcus neoformans
KW - Fluconazole
KW - Posaconazole
KW - Voriconazole
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U2 - 10.1046/j.1439-0507.2002.00765.x
DO - 10.1046/j.1439-0507.2002.00765.x
M3 - Article
C2 - 12421285
AN - SCOPUS:0036433022
VL - 45
SP - 378
EP - 383
JO - Mycoses
JF - Mycoses
SN - 0933-7407
IS - 9-10
ER -