In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.

B. A. Christy, G. A. Scangos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bovine papillomavirus (BPV) was methylated in vitro at either the 29 HpaII sites, the 27 HhaI sites, or both. Methylation of the HpaII sites reduced transformation by the virus two- to sixfold, while methylation at HhaI sites increased transformation two- to fourfold. DNA methylated at both HpaII and HhaI sites did not differ detectably from unmethylated DNA in its efficiency of transformation. These results indicate that specific methylation sites, rather than the absolute level of methylated cytosine residues, are important in determining the effects on transformation and that the negative effects of methylation at some sites can be compensated for by methylation at other sites. BPV molecules in cells transformed by methylated BPV DNA contained little or no methylation, indicating that the pattern of methylation was not faithfully retained in these extrachromosomally replicating molecules. Methylation at the HpaII sites (but not the HhaI sites) in the cloned BPV plasmid or in pBR322 also inhibited transformation of the plasmids into Escherichia coli HB101 cells.

Original languageEnglish (US)
Pages (from-to)2910-2915
Number of pages6
JournalMolecular and Cellular Biology
Volume6
Issue number8
StatePublished - Aug 1986
Externally publishedYes

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Methylation
DNA
Plasmids
In Vitro Techniques
Cytosine
Escherichia coli
Viruses

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells. / Christy, B. A.; Scangos, G. A.

In: Molecular and Cellular Biology, Vol. 6, No. 8, 08.1986, p. 2910-2915.

Research output: Contribution to journalArticle

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