In vitro maturation of oocytes derived from unstimulated antral follicles

R. G. Brzyski, C. A. Eddy

Research output: Contribution to journalArticlepeer-review


Background: Sixty years ago the first observations of in vitro human oocyte maturation were made. Today, the physiology of oocyte maturation remains mysterious. Gonadotropin stimulation has formed the foundation of contemporary assisted reproduction, but physicians, patients, and society have questioned the expense, complexity, and risk of gonadotropin therapy. Increasing demand for donor oocytes also has generated an increased interest in alternatives to current reproductive technologies. The authors reviewed recent investigations to identify promising trends for future development. Methods: Literature review was conducted based primarily on the use of the MEDLINE database, focusing on studies of human and nonhuman primate in vitro oocyte maturation in the past 5 years. Results: Cumulus-enclosed human oocytes derived from antral follicles in a variety of physiologic states, including pregnancy, have demonstrated the ability to mature in vitro, fertilize, divide, and result in the birth of liveborn offspring. Such development is compatible with a variety of culture conditions. Supplementation of culture media with gonadotropins and estradiol is used routinely, although the experimental basis for this is limited, Conclusions: Use of nonhuman primates helps establish research priorities in humans. The roles of growth factors, micromanipulation, and coculture are potentially rewarding areas for future investigation.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalAssisted Reproduction
Issue number2
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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