In vitro maturation of baboon oocytes retrieved at the time of cesarean section

Carlton A. Eddy, Robert G. Brzyski, Michelle M. Leland

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Objective: To investigate the feasibility of oocyte retrieval at the time of cesarean delivery and the potential of such oocytes to undergo nuclear maturation in vitro using a baboon model and an established culture system. Design: Randomized, controlled animal study. Setting: Research foundation and university research laboratory. Animal(s): Mature pregnant baboons. Intervention(s): In vitro culture of aspirated oocytes with or without epidermal growth factor (EGF). Main Outcome Measure(s): Oocyte yield, germinal vesicle breakdown, polar body extrusion. Result(s): A total of 246 oocytes were retrieved (mean, 35; range, 14-67). Eighty-seven oocytes (35%) underwent germinal vesicle breakdown and 72 oocytes (29%) extruded a polar body. A χ 2 analysis revealed no significant effect of EGF on outcome parameters. No effect of gestational age or maternal age on oocyte yield or development was observed. Conclusion(s): A sizeable proportion of oocytes obtained from puerperal primates exhibited the capacity to undergo nuclear maturation in vitro.

Original languageEnglish (US)
Pages (from-to)1153-1156
Number of pages4
JournalFertility and sterility
Volume71
Issue number6
DOIs
StatePublished - Jun 1999
Externally publishedYes

Keywords

  • Epidermal growth factor
  • Meiosis
  • Oocyte physiology

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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