In vitro inhibition of cholesterol synthesis by pregnenolone in bovine corpus luteum

Ajai Haksar; E. B. Romanoff; N. Hagino; G. Pincus

doi:10.1016/0039-128X(67)90028-1

In vitro inhibition of cholesterol synthesis by pregnenolone in bovine corpus luteum

Ajai Haksar, E. B. Romanoff, N. Hagino, G. Pincus

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Bovine corpus luteum slices and. minces were incubated with acetate-1-¹⁴C in the presence of pregnenolone. There was a general inhibition in the utilization of acetate-1-¹⁴C as manifested, by a decreased incorporation of radioactivity into digitonin precipitable sterols, cholesterol, progesterone, and the total lipid extract of the tissue. LH which alone would stimulate the incorporation of acetate-1-¹⁴C into these substances was not able to reverse the pregnenolone-induced. inhibition. It is suggested that pregnenolone may have a role in the regulation of steroidogenesis in the corpus luteum.

Original language	English (US)
Pages (from-to)	405-414
Number of pages	10
Journal	Steroids
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/0039-128X(67)90028-1
State	Published - Apr 1967
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/0039-128X(67)90028-1

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title = "In vitro inhibition of cholesterol synthesis by pregnenolone in bovine corpus luteum",

abstract = "Bovine corpus luteum slices and. minces were incubated with acetate-1-14C in the presence of pregnenolone. There was a general inhibition in the utilization of acetate-1-14C as manifested, by a decreased incorporation of radioactivity into digitonin precipitable sterols, cholesterol, progesterone, and the total lipid extract of the tissue. LH which alone would stimulate the incorporation of acetate-1-14C into these substances was not able to reverse the pregnenolone-induced. inhibition. It is suggested that pregnenolone may have a role in the regulation of steroidogenesis in the corpus luteum.",

author = "Ajai Haksar and Romanoff, {E. B.} and N. Hagino and G. Pincus",

year = "1967",

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pages = "405--414",

journal = "Steroids",

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T1 - In vitro inhibition of cholesterol synthesis by pregnenolone in bovine corpus luteum

AU - Haksar, Ajai

AU - Romanoff, E. B.

AU - Hagino, N.

AU - Pincus, G.

PY - 1967/4

Y1 - 1967/4

N2 - Bovine corpus luteum slices and. minces were incubated with acetate-1-14C in the presence of pregnenolone. There was a general inhibition in the utilization of acetate-1-14C as manifested, by a decreased incorporation of radioactivity into digitonin precipitable sterols, cholesterol, progesterone, and the total lipid extract of the tissue. LH which alone would stimulate the incorporation of acetate-1-14C into these substances was not able to reverse the pregnenolone-induced. inhibition. It is suggested that pregnenolone may have a role in the regulation of steroidogenesis in the corpus luteum.

AB - Bovine corpus luteum slices and. minces were incubated with acetate-1-14C in the presence of pregnenolone. There was a general inhibition in the utilization of acetate-1-14C as manifested, by a decreased incorporation of radioactivity into digitonin precipitable sterols, cholesterol, progesterone, and the total lipid extract of the tissue. LH which alone would stimulate the incorporation of acetate-1-14C into these substances was not able to reverse the pregnenolone-induced. inhibition. It is suggested that pregnenolone may have a role in the regulation of steroidogenesis in the corpus luteum.

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JO - Steroids

JF - Steroids

SN - 0039-128X

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ER -

In vitro inhibition of cholesterol synthesis by pregnenolone in bovine corpus luteum

Abstract

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