In vitro growth environment produces lipidomic and electron transport chain abnormalities in mitochondria from non-tumorigenic astrocytes and brain tumours

Michael A. Kiebish, Xianlin Han, Hua Cheng, Thomas N. Seyfried

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The mitochondrial lipidome influences ETC (electron transport chain) and cellular bioenergetic efficiency. Brain tumours are largely dependent on glycolysis for energy due to defects in mitochondria and oxidative phosphorylation. In the present study, we used shotgun lipidomics to compare the lipidome in highly purified mitochondria isolated from normal brain, from brain tumour tissue, from cultured tumour cells and from nontumorigenic astrocytes. The tumours included the CT-2A astrocytoma and an EPEN (ependymoblastoma), both syngeneic with the C57BL/6J (B6) mouse strain. The mitochondrial lipidome in cultured CT-2A and EPEN tumour cells were compared with those in cultured astrocytes and in solid tumours grown in vivo. Major differences were found between normal tissue and tumour tissue and between in vivo and in vitro growth environments for the content or composition of ethanolamine glycerophospholipids, phosphatidylglycerol and cardiolipin. The mitochondrial lipid abnormalities in solid tumours and in cultured cells were associated with reductions in multiple ETC activities, especially Complex I. The in vitro growth environment produced lipid and ETC abnormalities in cultured non-tumorigenic astrocytes that were similar to those associated with tumorigenicity. It appears that the culture environment obscures the boundaries of the Crabtree and the Warburg effects. These results indicate that in vitro growth environments can produce abnormalities in mitochondrial lipids and ETC activities, thus contributing to a dependency on glycolysis for ATP production.

Original languageEnglish (US)
Article numbere00011
Pages (from-to)125-138
Number of pages14
JournalASN Neuro
Volume1
Issue number3
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

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Electron Transport
Brain Neoplasms
Astrocytes
Mitochondria
Cultured Tumor Cells
Primitive Neuroectodermal Tumors
Glycolysis
Growth
Lipids
Neoplasms
Glycerophospholipids
Phosphatidylglycerols
Ethanolamine
Cardiolipins
Oxidative Phosphorylation
Astrocytoma
Firearms
Energy Metabolism
Adenosine Triphosphate
In Vitro Techniques

Keywords

  • Cancer
  • Culture
  • Glycolysis
  • Lipid
  • Metabolism
  • Shotgun lipidomics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

Cite this

In vitro growth environment produces lipidomic and electron transport chain abnormalities in mitochondria from non-tumorigenic astrocytes and brain tumours. / Kiebish, Michael A.; Han, Xianlin; Cheng, Hua; Seyfried, Thomas N.

In: ASN Neuro, Vol. 1, No. 3, e00011, 01.12.2009, p. 125-138.

Research output: Contribution to journalArticle

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