In vitro effect of an oral spray and mouthrinses on dual species cariogenic bacteria biofilm

Parveez Ahamed Abdul Azees, Hanzhou Wang, Xiao Dong Chen, Chih Ko Yeh, Franklin Garcia-Godoy

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To determine the efficacy of an oral spray and oral rinses to inhibit oral cariogenic dual species biofilm formation on hydroxyapatite (HA) discs. Methods: The Streptococcus mutans (NCTC 10449, ATCC), Lactobacilli easel (NCIB 8820, ATCC) dual species biofilm formation and inhibition on HA disc was tested using five antimicrobial products, i.e., oral spray (Oral Shield), Mouthrinse (Listerine Ultra Clean, Listerine Cool Mint, Crest Pro-Health, ACT Restoring). An untreated group served as control. The established biofilm on the surface of each disc was treated or untreated with oral spray and mouthrinse for 2 minutes after 24 or 48 hours. The dual species biofilm formation and inhibition on HA discs was determined using the spread plate method and colonies were counted and expressed as colony forming units (CFU/mL). Further, the HA disc was subjected to confocal laser scanning microscope (CLSM) examination to determine the viability of cells using live-dead staining and a scanning electron microscope (SEM) to examine the effect on bacteria biofilm and morphology. The cytotoxic effect of test spray and mouthrinse was tested on OKF6/TERT-2 cells using the MTT method. Results: At each time point, 24- or 48-hours, S. mutans and L. casei mixed biofilm on HA discs had a significantly (P> 0.001) fewer number of bacteria in the treated groups than the untreated one. The oral spray and mouthrinses had a detrimental effect on bacteria biofilm, morphology and cell wall, whereas no significant changes were observed in the untreated group. Cytotoxic assay revealed that the oral spray was safe for human oral keratinocyte cells.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalAmerican journal of dentistry
Volume35
Issue number2
StatePublished - Apr 2022

ASJC Scopus subject areas

  • Medicine(all)

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