In vitro effect of aflatoxin B1 on the transcriptional activity of DNA template, chromatin and soluble DNA-dependent RNA polymerases in buffalo liver

R. I. Mashaly; S. L. Habib; M. H. Salem; S. A. El Deeb; M. M. Safwat; F. Sarhan

doi:10.1016/0378-4274(88)90009-4

In vitro effect of aflatoxin B₁ on the transcriptional activity of DNA template, chromatin and soluble DNA-dependent RNA polymerases in buffalo liver

R. I. Mashaly, S. L. Habib, M. H. Salem, S. A. El Deeb, M. M. Safwat, F. Sarhan

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The effect of aflatoxin B₁ on the DNA template and DNA-dependent RNA polymerases in buffalo liver was studied. Aflatoxin B₁ inhibited both Mg²⁺- and Mn²⁺-activated RNA polymerases in a dosedependent manner. At 10 μg the inhibition of both enzymes was almost complete. The inhibitory effect on the solubilized enzymes was higher than the chromatin-bound, suggesting a direct effect at the enzyme level. On the other hand, incubating DNA or deoxyribonucleoprotein (DNP) with 2 μg aflatoxin reduces its transcriptional capacity with a greater effect on the Mg²⁺-activated RNA polymerase than the Mn²⁺-activated enzyme. These results suggest that aflatoxin B₁ inhibits in vitro transcription in buffalo liver at both enzyme and template levels.

Original language	English (US)
Pages (from-to)	69-75
Number of pages	7
Journal	Toxicology Letters
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/0378-4274(88)90009-4
State	Published - Apr 1988
Externally published	Yes

Keywords

Aflatoxin B
Buffalo liver
Chromatin
RNA polymerases

ASJC Scopus subject areas

Toxicology

Access to Document

10.1016/0378-4274(88)90009-4

Cite this

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title = "In vitro effect of aflatoxin B1 on the transcriptional activity of DNA template, chromatin and soluble DNA-dependent RNA polymerases in buffalo liver",

abstract = "The effect of aflatoxin B1 on the DNA template and DNA-dependent RNA polymerases in buffalo liver was studied. Aflatoxin B1 inhibited both Mg2+- and Mn2+-activated RNA polymerases in a dosedependent manner. At 10 μg the inhibition of both enzymes was almost complete. The inhibitory effect on the solubilized enzymes was higher than the chromatin-bound, suggesting a direct effect at the enzyme level. On the other hand, incubating DNA or deoxyribonucleoprotein (DNP) with 2 μg aflatoxin reduces its transcriptional capacity with a greater effect on the Mg2+-activated RNA polymerase than the Mn2+-activated enzyme. These results suggest that aflatoxin B1 inhibits in vitro transcription in buffalo liver at both enzyme and template levels.",

keywords = "Aflatoxin B, Buffalo liver, Chromatin, RNA polymerases",

author = "Mashaly, {R. I.} and Habib, {S. L.} and Salem, {M. H.} and {El Deeb}, {S. A.} and Safwat, {M. M.} and F. Sarhan",

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T1 - In vitro effect of aflatoxin B1 on the transcriptional activity of DNA template, chromatin and soluble DNA-dependent RNA polymerases in buffalo liver

AU - Mashaly, R. I.

AU - Habib, S. L.

AU - Salem, M. H.

AU - El Deeb, S. A.

AU - Safwat, M. M.

AU - Sarhan, F.

PY - 1988/4

Y1 - 1988/4

N2 - The effect of aflatoxin B1 on the DNA template and DNA-dependent RNA polymerases in buffalo liver was studied. Aflatoxin B1 inhibited both Mg2+- and Mn2+-activated RNA polymerases in a dosedependent manner. At 10 μg the inhibition of both enzymes was almost complete. The inhibitory effect on the solubilized enzymes was higher than the chromatin-bound, suggesting a direct effect at the enzyme level. On the other hand, incubating DNA or deoxyribonucleoprotein (DNP) with 2 μg aflatoxin reduces its transcriptional capacity with a greater effect on the Mg2+-activated RNA polymerase than the Mn2+-activated enzyme. These results suggest that aflatoxin B1 inhibits in vitro transcription in buffalo liver at both enzyme and template levels.

AB - The effect of aflatoxin B1 on the DNA template and DNA-dependent RNA polymerases in buffalo liver was studied. Aflatoxin B1 inhibited both Mg2+- and Mn2+-activated RNA polymerases in a dosedependent manner. At 10 μg the inhibition of both enzymes was almost complete. The inhibitory effect on the solubilized enzymes was higher than the chromatin-bound, suggesting a direct effect at the enzyme level. On the other hand, incubating DNA or deoxyribonucleoprotein (DNP) with 2 μg aflatoxin reduces its transcriptional capacity with a greater effect on the Mg2+-activated RNA polymerase than the Mn2+-activated enzyme. These results suggest that aflatoxin B1 inhibits in vitro transcription in buffalo liver at both enzyme and template levels.

KW - Aflatoxin B

KW - Buffalo liver

KW - Chromatin

KW - RNA polymerases

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