In vitro effect of aflatoxin B1 on the transcriptional activity of DNA template, chromatin and soluble DNA-dependent RNA polymerases in buffalo liver

R. I. Mashaly, S. L. Habib, M. H. Salem, S. A. El Deeb, M. M. Safwat, F. Sarhan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The effect of aflatoxin B1 on the DNA template and DNA-dependent RNA polymerases in buffalo liver was studied. Aflatoxin B1 inhibited both Mg2+- and Mn2+-activated RNA polymerases in a dosedependent manner. At 10 μg the inhibition of both enzymes was almost complete. The inhibitory effect on the solubilized enzymes was higher than the chromatin-bound, suggesting a direct effect at the enzyme level. On the other hand, incubating DNA or deoxyribonucleoprotein (DNP) with 2 μg aflatoxin reduces its transcriptional capacity with a greater effect on the Mg2+-activated RNA polymerase than the Mn2+-activated enzyme. These results suggest that aflatoxin B1 inhibits in vitro transcription in buffalo liver at both enzyme and template levels.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalToxicology Letters
Volume41
Issue number1
DOIs
StatePublished - Apr 1988
Externally publishedYes

Keywords

  • Aflatoxin B
  • Buffalo liver
  • Chromatin
  • RNA polymerases

ASJC Scopus subject areas

  • Toxicology

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