In vitro antifungal susceptibility and molecular identity of 99 clinical isolates of the opportunistic fungal genus Curvularia

Keith C. da Cunha, Deanna A. Sutton, Annette W. Fothergill, Josepa Gené, Josep Cano, Hugo Madrid, Sybren de Hoog, Pedro W. Crous, Josep Guarro

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The in vitro antifungal susceptibility of a set of 99 clinical isolates of Curvularia was tested against 9 drugs using a reference microdilution method. The isolates had been identified previously to species level by comparing their ITS rDNA and glyceraldehyde-3-phosphate dehydrogenase gene sequences with those of reference strains. We were able to reliably identify 73.2% of the isolates, the most frequent species being Curvularia aeria, Curvularia geniculata/. Curvularia senegalensis, Curvularia lunata, Curvularia inaequalis, Curvularia verruculosa, and Curvularia borreriae. Most of these isolates had been recovered from nasal sinus, which is generally considered one of the most frequent sites of infection by these fungi. In addition, at least 3 phylogenetic species that have not yet been formally described were detected. The most active drugs were the echinocandins, amphotericin B, and posaconazole, whereas voriconazole and itraconazole showed poor activity.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume76
Issue number2
DOIs
StatePublished - Jun 2013

Keywords

  • Antifungal susceptibility
  • Curvularia
  • GPDH gene
  • ITS region
  • Molecular identification

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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