In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouse

Wenfeng Zeng, Xiaozhe Yin, Yunhan Jiang, Lingtao Jin, Wei Liang

Research output: Contribution to journalArticlepeer-review

Abstract

Exosomes that contain various signaling molecules, such as proteins, nucleotides, metabolites, and lipids, are important for intercellular communication. Dendritic cells (DC) are central regulators of anti-tumor immunity but can be suppressed by tumor-derived exosomes (TDEs) in the tumor microenvironment. Here, we describe a step-by-step protocol for TDE isolation and evaluation of TDEs on DCs both in vitro and ex vivo with high repeatability. This approach is useful for the interrogating TDE-DC interactions and identification of novel immune regulators. For complete details on the use and execution of this protocol, please refer to Yin et al. (2020).

Original languageEnglish (US)
Article number100361
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021
Externally publishedYes

Keywords

  • Cancer
  • Cell-based assays
  • Flow cytometry/mass cytometry
  • Immunology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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