In vitro activity of eravacycline against common ribotypes of Clostridioides difficile

Eugénie Bassères, Khurshida Begum, Chris Lancaster, Anne J. Gonzales-Luna, Travis J. Carlson, Julie Miranda, Tasnuva Rashid, M. Jahangir Alam, David W. Eyre, Mark H. Wilcox, Kevin W. Garey

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background: Eravacycline is a novel synthetic fluorocycline antibacterial approved for complicated intraabdominal infections. Objectives: The purpose of this study was to assess the in vitro activities of eravacycline and comparator antibiotics against contemporary clinical isolates of Clostridioides difficile representing common ribotypes, including isolates with decreased susceptibility to metronidazole and vancomycin. Methods: Clinical C. difficile strains from six common or emerging ribotypes were used to test the in vitro activities of eravacycline and comparator antibiotics (fidaxomicin, vancomycin and metronidazole) by broth microdilution. In addition, MBC experiments, time-kill kinetic studies andWGS experiments were performed. Results: A total of 234 isolates were tested, including ribotypes RT001 (n = 37), RT002 (n = 41), RT014-020 (n = 39), RT027 (n = 42), RT106 (n = 38) and RT255 (n = 37). MIC50/90 values were lowest for eravacycline (_0.0078/0.016 mg/L), followed by fidaxomicin (0.016/0.063 mg/L), metronidazole (0.25/1.0 mg/L) and vancomycin (2.0/4.0 mg/L). MBCs were lower for eravacycline compared with vancomycin for all ribotypes tested. Both vancomycin and eravacycline demonstrated bactericidal killing, including for epidemic RT027. The presence of the tetM or tetW resistance genes did not affect the MIC of eravacycline. Conclusions: This study demonstrated potent in vitro activity of eravacycline against a large collection of clinical C. difficile strains that was not affected by ribotype, susceptibility to vancomycin or the presence of certain tet resistance genes. Further development of eravacycline as an antibiotic to be used in patients with Clostridioides difficile infection is warranted.

Original languageEnglish (US)
Pages (from-to)2879-2884
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume75
Issue number10
DOIs
StatePublished - Oct 1 2020
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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