In vitro activity of caspofungin against Candida albicans biofilms

Stefano P. Bachmann, Kacy VandeWalle, Gordon Ramage, Thomas F Patterson, Brian L Wickes, John R. Graybill, José L. López-Ribot

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Abstract

Most manifestations of candidiasis are associated with biofilm formation on biological or inanimate surfaces. Candida albicans biofilms are recalcitrant to treatment with conventional antifungal therapies. Here we report on the activity of caspofungin, a new semisynthetic echinocandin, against C. albicans biofilms. Caspofungin displayed potent in vitro activity against sessile C. albicans cells within biofilms, with MICs at which 50% of the sessile cells were inhibited well within the drug's therapeutic range. Scanning electron microscopy and confocal scanning laser microscopy were used to visualize the effects of caspofungin on preformed C. albicans biofilms, and the results indicated that caspofungin affected the cellular morphology and the metabolic status of cells within the biofilms. The coating of biomaterials with caspofungin had an inhibitory effect on subsequent biofilm development by C. albicans. Together these findings indicate that caspofungin displays potent activity against C. albicans biofilms in vitro and merits further investigation for the treatment of biofilm-associated infections.

Original languageEnglish (US)
Pages (from-to)3591-3596
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume46
Issue number11
DOIs
StatePublished - Nov 2002

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caspofungin
Biofilms
Candida albicans
In Vitro Techniques
Candidiasis
Biocompatible Materials
Confocal Microscopy
Electron Scanning Microscopy

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

In vitro activity of caspofungin against Candida albicans biofilms. / Bachmann, Stefano P.; VandeWalle, Kacy; Ramage, Gordon; Patterson, Thomas F; Wickes, Brian L; Graybill, John R.; López-Ribot, José L.

In: Antimicrobial Agents and Chemotherapy, Vol. 46, No. 11, 11.2002, p. 3591-3596.

Research output: Contribution to journalArticle

Bachmann, Stefano P. ; VandeWalle, Kacy ; Ramage, Gordon ; Patterson, Thomas F ; Wickes, Brian L ; Graybill, John R. ; López-Ribot, José L. / In vitro activity of caspofungin against Candida albicans biofilms. In: Antimicrobial Agents and Chemotherapy. 2002 ; Vol. 46, No. 11. pp. 3591-3596.
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AU - Graybill, John R.

AU - López-Ribot, José L.

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