In situ remineralisation of eroded enamel lesions by NaF rinses

Mary S. Mathews, Bennett T Amaechi, Karthikeyan Ramalingam, Renzo A. Ccahuana-Vasquez, Irene P. Chedjieu, Allen C. MacKey, Robert L. Karlinsey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: The purpose of this pilot study was to evaluate the remineralisation of eroded enamel by NaF rinses in an intra-oral model. Methods: Serving as their own control, subjects (N = 80) participated in a randomised, four-leg (20 subjects/leg), 28-day, parallel design study. In each leg, each participant wore a customised orthodontic bracket attached to a mandibular molar that contained one tooth block having an initial erosive lesion (0.3% citric acid, pH 3.75, 2 h). Within the 28-day period, participants engaged in twice-daily brushing for 1 min with a fluoride-free dentifrice followed by 1-min rinsing with one of the following aqueous rinses: fluoride-free (0 ppm F), 225 ppm F, 225 ppm F plus functionalised β-tricalcium phosphate (fTCP), and 450 ppm F. Following intra-oral exposure, appliances were removed and specimens were analysed using surface microhardness (SMH) and transverse microradiography (TMR). Results: Statistically significant (p < 0.05) remineralisation, as determined by SMH and TMR, of the eroded enamel relative to baseline occurred for each fluoride system. No significant differences in SMH were observed amongst the fluoride groups (p > 0.05), however, 225 ppm plus fTCP produced 27% and 7% SMH indent length reduction relative to 225 ppm F and 450 ppm F, respectively. No significant differences in TMR were observed amongst the fluoride groups (p > 0.05), however, 225 ppm F plus fTCP and 450 ppm F produced significant (p < 0.05) mineral gains relative to the fluoride-free control, whilst 225 ppm F did not (p > 0.05). Relative to the 225 ppm F group, the 450 ppm F and 225 ppm F plus fTCP groups produced 65% and 61% greater mineral change, respectively. Conclusions: These pilot results demonstrate this model is sensitive to fluoride and that addition of fTCP to an aqueous rinse containing 225 ppm F may provide significant remineralisation benefits. Therefore, the combination of relatively low levels of fluoride and fTCP might be an effective alternative to a high fluoride treatment for anti-erosion benefits.

Original languageEnglish (US)
Pages (from-to)525-530
Number of pages6
JournalArchives of Oral Biology
Volume57
Issue number5
DOIs
StatePublished - May 2012

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Dental Enamel
Fluorides
Microradiography
Leg
Orthodontic Brackets
Dentifrices
Citric Acid
Minerals
tricalcium phosphate
Tooth

Keywords

  • Enamel
  • Erosion
  • Fluoride
  • Remineralisation

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Mathews, M. S., Amaechi, B. T., Ramalingam, K., Ccahuana-Vasquez, R. A., Chedjieu, I. P., MacKey, A. C., & Karlinsey, R. L. (2012). In situ remineralisation of eroded enamel lesions by NaF rinses. Archives of Oral Biology, 57(5), 525-530. https://doi.org/10.1016/j.archoralbio.2011.10.010

In situ remineralisation of eroded enamel lesions by NaF rinses. / Mathews, Mary S.; Amaechi, Bennett T; Ramalingam, Karthikeyan; Ccahuana-Vasquez, Renzo A.; Chedjieu, Irene P.; MacKey, Allen C.; Karlinsey, Robert L.

In: Archives of Oral Biology, Vol. 57, No. 5, 05.2012, p. 525-530.

Research output: Contribution to journalArticle

Mathews, MS, Amaechi, BT, Ramalingam, K, Ccahuana-Vasquez, RA, Chedjieu, IP, MacKey, AC & Karlinsey, RL 2012, 'In situ remineralisation of eroded enamel lesions by NaF rinses' Archives of Oral Biology, vol. 57, no. 5, pp. 525-530. https://doi.org/10.1016/j.archoralbio.2011.10.010
Mathews MS, Amaechi BT, Ramalingam K, Ccahuana-Vasquez RA, Chedjieu IP, MacKey AC et al. In situ remineralisation of eroded enamel lesions by NaF rinses. Archives of Oral Biology. 2012 May;57(5):525-530. https://doi.org/10.1016/j.archoralbio.2011.10.010
Mathews, Mary S. ; Amaechi, Bennett T ; Ramalingam, Karthikeyan ; Ccahuana-Vasquez, Renzo A. ; Chedjieu, Irene P. ; MacKey, Allen C. ; Karlinsey, Robert L. / In situ remineralisation of eroded enamel lesions by NaF rinses. In: Archives of Oral Biology. 2012 ; Vol. 57, No. 5. pp. 525-530.
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abstract = "Objective: The purpose of this pilot study was to evaluate the remineralisation of eroded enamel by NaF rinses in an intra-oral model. Methods: Serving as their own control, subjects (N = 80) participated in a randomised, four-leg (20 subjects/leg), 28-day, parallel design study. In each leg, each participant wore a customised orthodontic bracket attached to a mandibular molar that contained one tooth block having an initial erosive lesion (0.3{\%} citric acid, pH 3.75, 2 h). Within the 28-day period, participants engaged in twice-daily brushing for 1 min with a fluoride-free dentifrice followed by 1-min rinsing with one of the following aqueous rinses: fluoride-free (0 ppm F), 225 ppm F, 225 ppm F plus functionalised β-tricalcium phosphate (fTCP), and 450 ppm F. Following intra-oral exposure, appliances were removed and specimens were analysed using surface microhardness (SMH) and transverse microradiography (TMR). Results: Statistically significant (p < 0.05) remineralisation, as determined by SMH and TMR, of the eroded enamel relative to baseline occurred for each fluoride system. No significant differences in SMH were observed amongst the fluoride groups (p > 0.05), however, 225 ppm plus fTCP produced 27{\%} and 7{\%} SMH indent length reduction relative to 225 ppm F and 450 ppm F, respectively. No significant differences in TMR were observed amongst the fluoride groups (p > 0.05), however, 225 ppm F plus fTCP and 450 ppm F produced significant (p < 0.05) mineral gains relative to the fluoride-free control, whilst 225 ppm F did not (p > 0.05). Relative to the 225 ppm F group, the 450 ppm F and 225 ppm F plus fTCP groups produced 65{\%} and 61{\%} greater mineral change, respectively. Conclusions: These pilot results demonstrate this model is sensitive to fluoride and that addition of fTCP to an aqueous rinse containing 225 ppm F may provide significant remineralisation benefits. Therefore, the combination of relatively low levels of fluoride and fTCP might be an effective alternative to a high fluoride treatment for anti-erosion benefits.",
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N2 - Objective: The purpose of this pilot study was to evaluate the remineralisation of eroded enamel by NaF rinses in an intra-oral model. Methods: Serving as their own control, subjects (N = 80) participated in a randomised, four-leg (20 subjects/leg), 28-day, parallel design study. In each leg, each participant wore a customised orthodontic bracket attached to a mandibular molar that contained one tooth block having an initial erosive lesion (0.3% citric acid, pH 3.75, 2 h). Within the 28-day period, participants engaged in twice-daily brushing for 1 min with a fluoride-free dentifrice followed by 1-min rinsing with one of the following aqueous rinses: fluoride-free (0 ppm F), 225 ppm F, 225 ppm F plus functionalised β-tricalcium phosphate (fTCP), and 450 ppm F. Following intra-oral exposure, appliances were removed and specimens were analysed using surface microhardness (SMH) and transverse microradiography (TMR). Results: Statistically significant (p < 0.05) remineralisation, as determined by SMH and TMR, of the eroded enamel relative to baseline occurred for each fluoride system. No significant differences in SMH were observed amongst the fluoride groups (p > 0.05), however, 225 ppm plus fTCP produced 27% and 7% SMH indent length reduction relative to 225 ppm F and 450 ppm F, respectively. No significant differences in TMR were observed amongst the fluoride groups (p > 0.05), however, 225 ppm F plus fTCP and 450 ppm F produced significant (p < 0.05) mineral gains relative to the fluoride-free control, whilst 225 ppm F did not (p > 0.05). Relative to the 225 ppm F group, the 450 ppm F and 225 ppm F plus fTCP groups produced 65% and 61% greater mineral change, respectively. Conclusions: These pilot results demonstrate this model is sensitive to fluoride and that addition of fTCP to an aqueous rinse containing 225 ppm F may provide significant remineralisation benefits. Therefore, the combination of relatively low levels of fluoride and fTCP might be an effective alternative to a high fluoride treatment for anti-erosion benefits.

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