In situ immune autoradiographic identification of cells in heart tissues of mice with coxsackievirus B3-induced myocarditis

E. K. Godeny, C. J. Gauntt

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

In adolescent CD-1 male mice inoculated with a myocarditic coxsackievirus B3 (CVB3(m)) acute focal lesions containing necrotic myocytes, infiltrating mononuclear cells, and fibroblasts developed. With the use of an in situ immune autoradiographic method with rat monoclonal antibodies (MAb) and an 35S-labeled antibody, viral antigen were detected outside of lesions. Macrophage, T lymphocytes, and natural killer (NK) cells were identified within myocarditic lesions during the acute phase of the disease. Macrophages detected by anti-Mac-1 MAb were in focal areas within myocarditic lesions on Days 4-7 after inoculation. T lymphocytes were detected in myocarditic lesions on Days 4-10, with MAb to Thy-1 and Lyt-1 antigens showing diffuse reaction patterns, suggesting random distribution of these cells in lesions. Focal areas of reactivity were detected with MAbs to L3T4 and Lyt-2 antigens, suggesting clusters of helper and cytotoxic/suppressor T lymphocytes, respectively. NK cells were presumptively detected by asialo GM1 surface marker in lesions at all times. The presence of activated NK cells in lesions was confirmed by assay of mechanically dissociated heart tissues on Day 8. These data describe the temporal sequence and identity of leukocytes entering into CVB3-induced focal myocarditic lesions during the acute phase of disease in CD-1 mice.

Original languageEnglish (US)
Pages (from-to)267-276
Number of pages10
JournalAmerican Journal of Pathology
Volume129
Issue number2
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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