In situ hybridization histochemistry: A technique for the study of gene expression in single cells

C. E. Gee; J. L. Roberts

doi:10.1089/dna.1983.2.157

In situ hybridization histochemistry: A technique for the study of gene expression in single cells

C. E. Gee, J. L. Roberts

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The patterns of growth hormone and proopiomelanocortin gene expression in the rat pituitary were used to detail the application of the in situ hybridization histochemistry technique in tissue sections. Parameters affecting the efficiency and resolution of hybridization have been optimized so that this technique can be used to identify sites of peptide synthesis and to study hormonal regulation of gene expression at the single cell level.

Original language	English (US)
Pages (from-to)	157-163
Number of pages	7
Journal	DNA
Volume	2
Issue number	2
DOIs	https://doi.org/10.1089/dna.1983.2.157
State	Published - Jan 1 1983
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.1089/dna.1983.2.157

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title = "In situ hybridization histochemistry: A technique for the study of gene expression in single cells",

abstract = "The patterns of growth hormone and proopiomelanocortin gene expression in the rat pituitary were used to detail the application of the in situ hybridization histochemistry technique in tissue sections. Parameters affecting the efficiency and resolution of hybridization have been optimized so that this technique can be used to identify sites of peptide synthesis and to study hormonal regulation of gene expression at the single cell level.",

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year = "1983",

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AB - The patterns of growth hormone and proopiomelanocortin gene expression in the rat pituitary were used to detail the application of the in situ hybridization histochemistry technique in tissue sections. Parameters affecting the efficiency and resolution of hybridization have been optimized so that this technique can be used to identify sites of peptide synthesis and to study hormonal regulation of gene expression at the single cell level.

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In situ hybridization histochemistry: A technique for the study of gene expression in single cells

Abstract

ASJC Scopus subject areas

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