In situ effect of a CPP-ACP chewing gum on enamel erosion associated or not with abrasion

Andressa Feitosa Bezerra de Oliveira, Luciana Vilar de Oliveira Diniz, Franklin Delano Soares Forte, Fabio Correia Sampaio, Renzo Alberto Ccahuana-Vásquez, Bennett Tochukwu Amaechi

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objectives: The purpose of this study is to analyze the in situ effect of a casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP) chewing gum on human enamel erosion lesion associated or not with abrasion. Material and methods: A three-way crossover study of 7 days was conducted involving 10 volunteers subjected to the same protocol: (G1) CPP-ACP sugar-free chewing gum, (G2) regular sugar-free chewing gum without CPP-ACP, and (G3) saliva—no chewing gum. An abrasion test was included in each phase. A 3D non-contact profilometry measurement of lesion depth and surface roughness was obtained of sound and eroded surfaces. A salivary calcium concentration was determined for all volunteers. ANOVA followed by Tukey’s test were used with a p < 0.05. Results: The enamel depth and the enamel surface roughness of the CPP-ACP gum group were significantly lower than the others (ANOVA, p < 0.05). No significant differences were observed between the treatments when associated with abrasion (p > 0.05). A positive and significant correlation was seen between the lesion depth and enamel surface roughness for GI (r = 0.87, p = 0.00) and GIII (r = 0.79, p = 0.00) groups. The estimated total calcium presented in the saliva after the chewed CPP-ACP gum showed no statistical significance between the mean absorbance values at the different time collections (p > 0.05). Conclusions: It is demonstrated that the incorporation of the CPP-ACP into a sugar-free gum significantly increased the remineralization/protection of eroded enamel surface. Clinical relevance: The CPP-ACP added to gum may be a suitable alternative vehicle, to deliver calcium ions to saliva and therefore protecting enamel.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalClinical Oral Investigations
Issue number1
StatePublished - Jan 1 2017


  • Calcium phosphate
  • Casein phosphopeptide-amorphous
  • Enamel
  • Erosion

ASJC Scopus subject areas

  • Dentistry(all)


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