In silico analysis of IRES RNAs of foot-and-mouth disease virus and related picornaviruses

Jody M. Burks, Christian W Zwieb, Florian Müller, Iwona K. Wower, Jacek Wower

Research output: Contribution to journalArticle

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Abstract

Foot-and-mouth disease virus (FMDV) uses an internal ribosome entry site (IRES), a highly structured segment of its genomic RNA, to hijack the translational apparatus of an infected host. Computational analysis of 162 type II picornavirus IRES RNA sequences yielded secondary structures that included only base pairs supported by comparative or experimental evidence. The deduced helical sections provided the foundation for a hypothetical three-dimensional model of FMDV IRES RNA. The model was further constrained by incorporation of data derived from chemical modification and enzymatic probing of IRES RNAs as well as high-resolution information about IRES RNA-bound proteins.

Original languageEnglish (US)
Pages (from-to)1737-1747
Number of pages11
JournalArchives of Virology
Volume156
Issue number10
DOIs
StatePublished - Oct 2011

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Picornaviridae
Foot-and-Mouth Disease Virus
Computer Simulation
RNA
Base Pairing
Internal Ribosome Entry Sites
Proteins

ASJC Scopus subject areas

  • Virology

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In silico analysis of IRES RNAs of foot-and-mouth disease virus and related picornaviruses. / Burks, Jody M.; Zwieb, Christian W; Müller, Florian; Wower, Iwona K.; Wower, Jacek.

In: Archives of Virology, Vol. 156, No. 10, 10.2011, p. 1737-1747.

Research output: Contribution to journalArticle

Burks, Jody M. ; Zwieb, Christian W ; Müller, Florian ; Wower, Iwona K. ; Wower, Jacek. / In silico analysis of IRES RNAs of foot-and-mouth disease virus and related picornaviruses. In: Archives of Virology. 2011 ; Vol. 156, No. 10. pp. 1737-1747.
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