Improvements in procedures for electrophoresis in dilute agarose gels

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Procedures have been developed for performing electrophoresis in agarose gels with agarose concentrations as low as 0.035%. Using these procedures, agarose gel electrophoresis of the following has been performed: (a) bacteriophage T7 missing its tail fibers; no detectable sieving of this spherical particle (radius = 30.1 nm) occurred below 0.075% agarose, (b) duplex DNAs with molecular weights between 26.5 × 106 and 110 × 106.

Original languageEnglish (US)
Pages (from-to)351-356
Number of pages6
JournalAnalytical Biochemistry
Volume112
Issue number2
DOIs
Publication statusPublished - Apr 1981

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this