The accuracy of electrophoretic mobility (μ) determinations made by electrophoresis in gels is limited by the accuracy of the control of voltage gradient and temperature. To improve the accuracy of μ determinations during electrophoresis in horizontal slab gels, apparatuses have been built and procedures have been developed for improved control of the voltage gradient and temperature during electrophoresis. Temperature is controlled ± 0.2–0.3°C and voltage gradient is controlled ± 1–2%.
ASJC Scopus subject areas
- Clinical Biochemistry