Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins

David L. Rainwater, Perry H. Moore, Israel O. Gamboa

    Research output: Contribution to journalArticle

    19 Scopus citations

    Abstract

    Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a laboratory-made gel. To help overcome this limitation, we recommend a modification-addition of a sucrose gradient-that significantly improves within-gel variation in protein mobility.

    Original languageEnglish (US)
    Pages (from-to)773-775
    Number of pages3
    JournalJournal of lipid research
    Volume45
    Issue number4
    DOIs
    StatePublished - Apr 2004

    Keywords

    • Baboon
    • Gradient gel electrophoresis
    • Human
    • Lipoprotein separation

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology
    • Cell Biology

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