Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins

David L. Rainwater, Perry H. Moore, Israel O. Gamboa

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a laboratory-made gel. To help overcome this limitation, we recommend a modification-addition of a sucrose gradient-that significantly improves within-gel variation in protein mobility.

Original languageEnglish (US)
Pages (from-to)773-775
Number of pages3
JournalJournal of lipid research
Volume45
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

Keywords

  • Baboon
  • Gradient gel electrophoresis
  • Human
  • Lipoprotein separation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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