Improved isolation of undersampled bacteriophages: Finding of distant terminase genes

Philip Serwer, Shirley J. Hayes, Shehnaz Zaman, Karen Lieman, Mandy Rolando, Stephen C. Hardies

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Isolation and characterization of new environmental bacteriophages are performed for (1) analyzing microbial evolution and ecology and (2) delivering biological therapy. The sampling of environmental bacteriophages appears, however, to be limited by the procedure (usually liquid enrichment culture) used to propagate them. An alternative, less competitive procedure is developed here for the purpose of isolating new bacteriophages. This procedure involves extraction directly into and then propagation in a dilute agarose gel. Adaptations of this procedure are used to avoid repeated isolation of the same bacteriophage. Some newly isolated bacteriophages grow so poorly that they appear inaccessible to liquid enrichment culture. Four comparatively high titer bacteriophages were isolated and characterized by a genomic sequence survey. Some had genomes with extremely distant relationships to those of other bacteriophages, based on a tree built from the large terminase genes. These methods find novel genomes by rapidly isolating and screening diverse bacteriophages.

Original languageEnglish (US)
Pages (from-to)412-424
Number of pages13
JournalVirology
Volume329
Issue number2
DOIs
StatePublished - Nov 24 2004

Keywords

  • Agarose
  • Bacteriophage 1102φ3-1
  • Environmental bacteria
  • Genomic nucleotide sequence
  • Nucleotide sequence homology

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Improved isolation of undersampled bacteriophages: Finding of distant terminase genes'. Together they form a unique fingerprint.

Cite this