Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease

Marc D Feldman, Carlos R. Ayers, Marcia R. Lehman, Heidi E. Taylor, Vicki L. Gordon, Peter J. Sabia, Don Ras, Thomas C. Skalak, Joel Linden

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Attempts to monitor coronary sinus adenosine as a clinical marker of myocardial ischemia in humans have been disappointing. Accordingly, procedures have been developed for detecting adenosine in blood collected from the human coronary sinus. Collection involves using a double-lumen metabolic catheter, which allows blood to be mixed with a stop solution at the catheter tip, thereby minimizing adenosine formation and degradation. A five-component stop solution almost completely arrests adenosine formation and degradation. Adenosine analysis is improved by using both boronate and C18 Sep-Pak columns to purify and concentrate adenosine in human plasma before HPLC. Plasma adenosine in the coronary sinus of patients with and without coronary artery disease, measured before and during peak atrial pacing, showed a twofold atrial pacing-induced increase in adenosine in the patients with coronary artery disease (n = 9, P <0.001) but no change in the patients with normal epicardial coronary arteries (n = 6). These preliminary results indicate that coronary sinus adenosine may provide an index of myocardial ischemia in patients with coronary artery disease.

Original languageEnglish (US)
Pages (from-to)256-262
Number of pages7
JournalClinical Chemistry
Volume38
Issue number2
StatePublished - Feb 1992
Externally publishedYes

Fingerprint

Coronary Sinus
Adenosine
Coronary Artery Disease
Ischemia
Catheters
Myocardial Ischemia
Blood
Plasma (human)
Degradation
Coronary Vessels
Biomarkers
High Pressure Liquid Chromatography
Plasmas

Keywords

  • Chromatography, liquid
  • Metabolic catheter
  • Myocardial ischemia

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Feldman, M. D., Ayers, C. R., Lehman, M. R., Taylor, H. E., Gordon, V. L., Sabia, P. J., ... Linden, J. (1992). Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease. Clinical Chemistry, 38(2), 256-262.

Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease. / Feldman, Marc D; Ayers, Carlos R.; Lehman, Marcia R.; Taylor, Heidi E.; Gordon, Vicki L.; Sabia, Peter J.; Ras, Don; Skalak, Thomas C.; Linden, Joel.

In: Clinical Chemistry, Vol. 38, No. 2, 02.1992, p. 256-262.

Research output: Contribution to journalArticle

Feldman, MD, Ayers, CR, Lehman, MR, Taylor, HE, Gordon, VL, Sabia, PJ, Ras, D, Skalak, TC & Linden, J 1992, 'Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease', Clinical Chemistry, vol. 38, no. 2, pp. 256-262.
Feldman, Marc D ; Ayers, Carlos R. ; Lehman, Marcia R. ; Taylor, Heidi E. ; Gordon, Vicki L. ; Sabia, Peter J. ; Ras, Don ; Skalak, Thomas C. ; Linden, Joel. / Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease. In: Clinical Chemistry. 1992 ; Vol. 38, No. 2. pp. 256-262.
@article{3b97190a00994708b05cf887de436d50,
title = "Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease",
abstract = "Attempts to monitor coronary sinus adenosine as a clinical marker of myocardial ischemia in humans have been disappointing. Accordingly, procedures have been developed for detecting adenosine in blood collected from the human coronary sinus. Collection involves using a double-lumen metabolic catheter, which allows blood to be mixed with a stop solution at the catheter tip, thereby minimizing adenosine formation and degradation. A five-component stop solution almost completely arrests adenosine formation and degradation. Adenosine analysis is improved by using both boronate and C18 Sep-Pak columns to purify and concentrate adenosine in human plasma before HPLC. Plasma adenosine in the coronary sinus of patients with and without coronary artery disease, measured before and during peak atrial pacing, showed a twofold atrial pacing-induced increase in adenosine in the patients with coronary artery disease (n = 9, P <0.001) but no change in the patients with normal epicardial coronary arteries (n = 6). These preliminary results indicate that coronary sinus adenosine may provide an index of myocardial ischemia in patients with coronary artery disease.",
keywords = "Chromatography, liquid, Metabolic catheter, Myocardial ischemia",
author = "Feldman, {Marc D} and Ayers, {Carlos R.} and Lehman, {Marcia R.} and Taylor, {Heidi E.} and Gordon, {Vicki L.} and Sabia, {Peter J.} and Don Ras and Skalak, {Thomas C.} and Joel Linden",
year = "1992",
month = "2",
language = "English (US)",
volume = "38",
pages = "256--262",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "2",

}

TY - JOUR

T1 - Improved detection of ischemia-induced increases in coronary sinus adenosine in patients with coronary artery disease

AU - Feldman, Marc D

AU - Ayers, Carlos R.

AU - Lehman, Marcia R.

AU - Taylor, Heidi E.

AU - Gordon, Vicki L.

AU - Sabia, Peter J.

AU - Ras, Don

AU - Skalak, Thomas C.

AU - Linden, Joel

PY - 1992/2

Y1 - 1992/2

N2 - Attempts to monitor coronary sinus adenosine as a clinical marker of myocardial ischemia in humans have been disappointing. Accordingly, procedures have been developed for detecting adenosine in blood collected from the human coronary sinus. Collection involves using a double-lumen metabolic catheter, which allows blood to be mixed with a stop solution at the catheter tip, thereby minimizing adenosine formation and degradation. A five-component stop solution almost completely arrests adenosine formation and degradation. Adenosine analysis is improved by using both boronate and C18 Sep-Pak columns to purify and concentrate adenosine in human plasma before HPLC. Plasma adenosine in the coronary sinus of patients with and without coronary artery disease, measured before and during peak atrial pacing, showed a twofold atrial pacing-induced increase in adenosine in the patients with coronary artery disease (n = 9, P <0.001) but no change in the patients with normal epicardial coronary arteries (n = 6). These preliminary results indicate that coronary sinus adenosine may provide an index of myocardial ischemia in patients with coronary artery disease.

AB - Attempts to monitor coronary sinus adenosine as a clinical marker of myocardial ischemia in humans have been disappointing. Accordingly, procedures have been developed for detecting adenosine in blood collected from the human coronary sinus. Collection involves using a double-lumen metabolic catheter, which allows blood to be mixed with a stop solution at the catheter tip, thereby minimizing adenosine formation and degradation. A five-component stop solution almost completely arrests adenosine formation and degradation. Adenosine analysis is improved by using both boronate and C18 Sep-Pak columns to purify and concentrate adenosine in human plasma before HPLC. Plasma adenosine in the coronary sinus of patients with and without coronary artery disease, measured before and during peak atrial pacing, showed a twofold atrial pacing-induced increase in adenosine in the patients with coronary artery disease (n = 9, P <0.001) but no change in the patients with normal epicardial coronary arteries (n = 6). These preliminary results indicate that coronary sinus adenosine may provide an index of myocardial ischemia in patients with coronary artery disease.

KW - Chromatography, liquid

KW - Metabolic catheter

KW - Myocardial ischemia

UR - http://www.scopus.com/inward/record.url?scp=0026524789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026524789&partnerID=8YFLogxK

M3 - Article

C2 - 1541009

AN - SCOPUS:0026524789

VL - 38

SP - 256

EP - 262

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 2

ER -