Improved assays for hydralazine and hydralazine pyruvic acid hydrazone in human plasma

T. M. Ludden; L. K. Ludden; J. L. McNay; H. B. Skrdlant; P. J. Swaggerty; A. M.M. Shepherd

doi:10.1016/S0003-2670(01)84373-7

Improved assays for hydralazine and hydralazine pyruvic acid hydrazone in human plasma

T. M. Ludden, L. K. Ludden, J. L. McNay, H. B. Skrdlant, P. J. Swaggerty, A. M.M. Shepherd

Division of General Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Previous determinations of hydralazine and hydralazine pyruvic acid hydrazone based on derivatization and high-performance liquid chromatography have been modified to improve the detection limits of the hydralazine assay and the selectivity of the hydralazine pyruvic acid hydrazone technique. The lower limits of determinations of hydralazine and hydralazine pyruvic acid hydrazone are 2 and 25 ng ml^-1, respectively. The application of this technique to the determination of these substances in human plasma is demonstrated.

Original language	English (US)
Pages (from-to)	297-304
Number of pages	8
Journal	Analytica Chimica Acta
Volume	120
DOIs	https://doi.org/10.1016/S0003-2670(01)84373-7
State	Published - 1980

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Environmental Chemistry

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10.1016/S0003-2670(01)84373-7

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@article{96276a5699804078bf3d27061030d58d,

title = "Improved assays for hydralazine and hydralazine pyruvic acid hydrazone in human plasma",

abstract = "Previous determinations of hydralazine and hydralazine pyruvic acid hydrazone based on derivatization and high-performance liquid chromatography have been modified to improve the detection limits of the hydralazine assay and the selectivity of the hydralazine pyruvic acid hydrazone technique. The lower limits of determinations of hydralazine and hydralazine pyruvic acid hydrazone are 2 and 25 ng ml-1, respectively. The application of this technique to the determination of these substances in human plasma is demonstrated.",

author = "Ludden, {T. M.} and Ludden, {L. K.} and McNay, {J. L.} and Skrdlant, {H. B.} and Swaggerty, {P. J.} and Shepherd, {A. M.M.}",

note = "Funding Information: This work was supported by grants from the Texas Affiliate, Inc., the American Heart Association and NIH grant GM24092. We thank Ms. Rarbara Boyle and Ms. Beverly Keeton for their help in preparing this paper, and Drs. W. Reiss and K. Schmid (Ciba-Geigy, Ltd., Base& Switzerland) for their generous gift of authentic 4-(2-acetylhydrazino)phthalazinone.",

year = "1980",

doi = "10.1016/S0003-2670(01)84373-7",

language = "English (US)",

volume = "120",

pages = "297--304",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

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TY - JOUR

T1 - Improved assays for hydralazine and hydralazine pyruvic acid hydrazone in human plasma

AU - Ludden, T. M.

AU - Ludden, L. K.

AU - McNay, J. L.

AU - Skrdlant, H. B.

AU - Swaggerty, P. J.

AU - Shepherd, A. M.M.

N1 - Funding Information: This work was supported by grants from the Texas Affiliate, Inc., the American Heart Association and NIH grant GM24092. We thank Ms. Rarbara Boyle and Ms. Beverly Keeton for their help in preparing this paper, and Drs. W. Reiss and K. Schmid (Ciba-Geigy, Ltd., Base& Switzerland) for their generous gift of authentic 4-(2-acetylhydrazino)phthalazinone.

PY - 1980

Y1 - 1980

N2 - Previous determinations of hydralazine and hydralazine pyruvic acid hydrazone based on derivatization and high-performance liquid chromatography have been modified to improve the detection limits of the hydralazine assay and the selectivity of the hydralazine pyruvic acid hydrazone technique. The lower limits of determinations of hydralazine and hydralazine pyruvic acid hydrazone are 2 and 25 ng ml-1, respectively. The application of this technique to the determination of these substances in human plasma is demonstrated.

AB - Previous determinations of hydralazine and hydralazine pyruvic acid hydrazone based on derivatization and high-performance liquid chromatography have been modified to improve the detection limits of the hydralazine assay and the selectivity of the hydralazine pyruvic acid hydrazone technique. The lower limits of determinations of hydralazine and hydralazine pyruvic acid hydrazone are 2 and 25 ng ml-1, respectively. The application of this technique to the determination of these substances in human plasma is demonstrated.

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U2 - 10.1016/S0003-2670(01)84373-7

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SN - 0003-2670

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SP - 297

EP - 304

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

ER -

Improved assays for hydralazine and hydralazine pyruvic acid hydrazone in human plasma

Abstract

ASJC Scopus subject areas

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