Impaired proliferation response after PDGF induction in fibroblasts from Hutchinson-Guilford Progeria Syndrome

J. B. Stachniak, Z. D. Sharp, T. B. Pool, I. L. Cameron

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Fibroblasts from a Hutchinson-Guilford Progeria Syndrome (HGPS) patient were compared to normal human fibroblasts to determine if differences existed in growth factor mediated cell proliferation. Cultures of progeric fibroblasts were exposed individually to platelet-derived growth factor (PDGF), epidermal growth factor (EGF), platelet poor plasma (PPP) and fetal bovine serum (FBS). Autoradiographic studies using 3H thymidine showed that progeric fibroblasts had similar labeling indices relative to controls after exposure to FBS and EGF. In contrast, progeric cells made competent with PDGF and later treated with 5% PPP had a significantly lower labeling index. This and preliminary observations on fos RNA accumulation suggests the possible existence of a genetic defect in HGPS fibroblasts.

Original languageEnglish (US)
Pages (from-to)621-626
Number of pages6
JournalCell Biology International Reports
Volume12
Issue number8
DOIs
StatePublished - Aug 1988

ASJC Scopus subject areas

  • Cell Biology

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